PMID- 16529758
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1118
IP  - 1
DP  - 2006 Jun 16
TI  - Direct amino acid analysis method for speciation of selenoamino acids using 
      high-performance anion-exchange chromatography coupled with integrated pulsed 
      amperometric detection.
PG  - 134-8
AB  - Speciation analysis of selenomethylcysteine (SeMeCys), selenomethionine (SeMet) 
      and selenocystine (SeCys) has been performed using a direct amino acid analysis 
      method with high-performance anion-exchange chromatography (HPAEC) coupled with 
      integrated pulsed amperometric detection (IPAD). Three selenoamino acids could be 
      baseline-separated from 19 amino acids using gradient elution conditions for 
      amino acids and determined under new six-potential waveform. Detection limits for 
      SeMeCys, SeMet and SeCys were 0.25, 1 and 20 microg/L (25 microL injection, 10 
      times of the baseline noise), respectively. The relative standard deviations 
      (RSDs) of 200 microg/L SeMeCys, SeMet and SeCys were 3.1, 4.1 and 2.8%, 
      respectively (n=9, 25 microL injection). The proposed method has been applied for 
      determination of selenoamino acids in extracts of garlic and selenious yeast 
      granule samples. No selenoamino acids were found in garlic. Both SeMet and SeCys 
      were detected in selenious yeast tablet with the content of 45 and 129 microg 
      Se/g, respectively. Selenoamino acids standards were spiked in garlic and yeast 
      granule samples and the recovery ranged from 90 to 106%.
FAU - Liang, Lina
AU  - Liang L
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 
      2871, Beijing 100085, China.
FAU - Mo, Shumin
AU  - Mo S
FAU - Cai, Yaqi
AU  - Cai Y
FAU - Mou, Shifen
AU  - Mou S
FAU - Jiang, Guibin
AU  - Jiang G
FAU - Wen, Meijuan
AU  - Wen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060310
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Amino Acids)
RN  - 0 (Anion Exchange Resins)
RN  - 0 (Organoselenium Compounds)
RN  - 1464-43-3 (selenocystine)
RN  - 48TCX9A1VT (Cystine)
RN  - 964MRK2PEL (Selenomethionine)
SB  - IM
MH  - Amino Acids/*analysis/isolation & purification
MH  - Anion Exchange Resins/*chemistry
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Cystine/analogs & derivatives/analysis/isolation & purification
MH  - Electrochemistry/instrumentation/*methods
MH  - Garlic/chemistry
MH  - Organoselenium Compounds/analysis/isolation & purification
MH  - Reproducibility of Results
MH  - Selenomethionine/analysis/isolation & purification
MH  - Yeasts/chemistry
EDAT- 2006/03/15 09:00
MHDA- 2006/08/25 09:00
CRDT- 2006/03/15 09:00
PHST- 2005/10/11 00:00 [received]
PHST- 2006/02/17 00:00 [revised]
PHST- 2006/02/17 00:00 [accepted]
PHST- 2006/03/15 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2006/03/15 09:00 [entrez]
AID - S0021-9673(06)00405-5 [pii]
AID - 10.1016/j.chroma.2006.02.043 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Jun 16;1118(1):134-8. doi: 10.1016/j.chroma.2006.02.043. 
      Epub 2006 Mar 10.