PMID- 1653159
OWN - NLM
STAT- MEDLINE
DCOM- 19911010
LR  - 20190907
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 16
IP  - 4
DP  - 1991 May
TI  - Evaluation of changes in the secretion of immunoactive inhibin by adult rat 
      seminiferous tubules in vitro as an indicator of early toxicant action on 
      spermatogenesis.
PG  - 710-24
AB  - A method for culturing isolated seminiferous tubules (ST) from adult rats for 1-3 
      days has been developed and optimized rigorously on the basis of the secretion of 
      immunoactive inhibin under basal conditions and after maximal stimulation with 
      rat FSH or dibutyryl cyclic AMP. The effect on these cultures of three known 
      testicular toxicants was assessed. Of these, two are thought to act on the 
      Sertoli cell, meta-dinitrobenzene (mDNB) and nitrobenzene (NB), while the third, 
      methoxy acetic acid (MAA), is thought to act on pachytene spermatocytes. In 
      addition, the effect of a possible testicular toxicant, 3-mononitrotoluene 
      (3-MNT), was investigated. These data were compared with those obtained using 
      cultures of immature rat Sertoli cells (SC) or SC + germ cells and with data on 
      the effect of equivalent doses of the compounds on the secretion of immunoactive 
      inhibin in vivo. In studies designed to optimize conditions for the secretion of 
      immunoactive inhibin by ST in culture, significant effects were found of the type 
      of culture medium used, the duration of culture, the total and individual length 
      of tubules used, etc. All subsequent studies with toxicants utilized optimal 
      conditions. Addition of either mDNB or NB to ST cultures at 10(-5) or 10(-3) M, 
      or MAA at 10(-4) M, stimulated basal secretion of immunoactive inhibin by two- to 
      fourfold on Days 1, 2, or 3 of culture while FSH or dibutyryl cyclic 
      AMP-stimulated secretion of immunoactive inhibin was either unaffected or was 
      enhanced to a small extent. At the same doses, mDNB or NB also enhanced secretion 
      of immunoactive inhibin by SC cultures. although these effects were more variable 
      and of smaller magnitude than the effects on ST cultures. In contrast, addition 
      of up to 10(-3) MAA to cocultures of SC + germ cells had no effect on the 
      secretion of immunoactive inhibin. Exposure of rats in vivo to levels of mDNB, 
      NB, or MAA similar to those which stimulated secretion of immunoactive inhibin in 
      vitro resulted in a two- to fourfold increase in the levels of immunoactive 
      inhibin in testicular interstitial fluid (IF) at 1 and 3 days post-treatment, and 
      this was associated with early impairment of spermatogenesis (as judged by testis 
      weight). In contrast to these effects, addition of 3-MNT to ST or SC cultures had 
      no effect except at 10(-3) M, when the secretion of immunoactive inhibin was 
      increased marginally.(ABSTRACT TRUNCATED AT 400 WORDS)
FAU - Allenby, G
AU  - Allenby G
AD  - MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, United 
      Kingdom.
FAU - Foster, P M
AU  - Foster PM
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Acetates)
RN  - 0 (Culture Media)
RN  - 0 (Dinitrobenzenes)
RN  - 29A9W826KQ (3-nitrotoluene)
RN  - 3FPU23BG52 (Toluene)
RN  - 57285-09-3 (Inhibins)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - DK8B627BU0 (3-dinitrobenzene)
RN  - F11T1H7Q7W (methoxyacetic acid)
SB  - IM
MH  - Acetates/toxicity
MH  - Animals
MH  - Body Fluids/drug effects/physiology
MH  - Bucladesine/metabolism
MH  - Culture Media
MH  - Culture Techniques
MH  - Dinitrobenzenes/toxicity
MH  - Evaluation Studies as Topic
MH  - Follicle Stimulating Hormone/metabolism
MH  - Germ Cells/drug effects
MH  - Inhibins/immunology/*metabolism
MH  - Male
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Seminiferous Tubules/drug effects/*metabolism
MH  - Sertoli Cells/drug effects
MH  - Spermatogenesis/*drug effects
MH  - Testis/drug effects/physiology
MH  - Toluene/analogs & derivatives/toxicity
MH  - Toxicology/*methods
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
AID - 10.1016/0272-0590(91)90157-y [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1991 May;16(4):710-24. doi: 10.1016/0272-0590(91)90157-y.