PMID- 16532969
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20060314
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 26
IP  - 10
DP  - 2005 Oct
TI  - [Effect of brain-derived neurotrophic factor in human myeloma cells on 
      angiogenesis].
PG  - 602-6
AB  - OBJECTIVE: To investigate the expression of brain-derived neurotrophic factor 
      (BDNF) in multiple myeloma (MM) cells and the correlation between BDNF and MM 
      angiogenesis. METHODS: The expressions of BDNF mRNA transcripts and protein in MM 
      cell lines (RPMI 8226, KM3) were determined by RT-PCR and Western blot, 
      respectively, BDNF levels in culture supernatant by enzyme-linked immunosorbent 
      assay. Proliferation of human umbilical vein endothelial cells (HUVEC) mixed with 
      MM culture medium at different concentrations was examined by MTT assay. The 
      effects of MM culture medium on HUVEC migration and tube formation were studied 
      by modified Boyden chamber assay and tube formation assay, respectively. RESULTS: 
      BDNF was expressed in and secreted by MM cell lines RPMI 8226 and KM3. BDNF 
      concentrations in culture supernatants were within the range of its biological 
      activity. MM culture medium induced a concentration-dependent proliferation of 
      HUVEC. The number of HUVEC at a concentration of 50% KM3 culture medium and at 
      full KM3 culture medium were (1.85 +/- 0.23)-fold and (2.16 +/- 0.29) -fold 
      increase, respectively (P <0.05), compared with that of control. The 
      proliferative activity of HUVEC was reduced on the addition of BDNF antibody to 
      the culture medium. MM culture medium also stimulated the migration and 
      differentiation of HUVEC in vitro, the chemotactic index of HUVEC at a 
      concentration of 50% KM3 culture medium and at full KM3 culture medium were 1.85 
      +/- 0.23 and 2.16 +/- 0.29, respectively (P < 0.05). Full KM3 culture medium also 
      stimulated capillary-like tube formation in HUVEC (P <0.01), and addition of 
      anti-human BDNF antibody neutralized these effects significantly. CONCLUSION: MM 
      cell lines expressed and secreted biologically active BDNF, which may be 
      involved, at least in part, in MM angiogenesis.
FAU - Hu, Yu
AU  - Hu Y
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Wu, Tao
AU  - Wu T
FAU - Wang, Ya-Dan
AU  - Wang YD
FAU - He, Wen-Juan
AU  - He WJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism/pharmacology
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Endothelial Cells/drug effects/physiology
MH  - Humans
MH  - Multiple Myeloma/blood supply/*metabolism
MH  - *Neovascularization, Pathologic/metabolism
MH  - RNA, Messenger/genetics
EDAT- 2006/03/15 09:00
MHDA- 2009/05/08 09:00
CRDT- 2006/03/15 09:00
PHST- 2006/03/15 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2006/03/15 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2005 Oct;26(10):602-6.