PMID- 16534498
OWN - NLM
STAT- MEDLINE
DCOM- 20060627
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 86
IP  - 5
DP  - 2006 May
TI  - A potential role of somatostatin and its receptor SSTR4 in the migration of 
      hepatic oval cells.
PG  - 477-89
AB  - Somatostatin (SST) is a regulatory peptide that activates G protein-coupled 
      receptors comprised of five members (somatostatin receptors (SSTRs) 1-5). Despite 
      the broad use of SST and its analogs in clinical practice, the spectrum of SST 
      activities has been incompletely defined. Recently, it has been demonstrated that 
      SST can be a chemoattractant for hematopoietic precursor cells. Since hepatic 
      oval cells (HOCs) share common characteristics with hematopoietic stem cells, we 
      hypothesized that SST could act as a chemoattractant for HOCs by stimulating 
      SSTRs. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot 
      assay revealed an increased expression of SST in the 2-acetyl-aminofluorene 
      (2AAF)/partial hepatectomy (PHx) HOC induction model. Immunohistochemical 
      staining showed the expression of SST in 2AAF/PHx-treated rat liver, as compared 
      to normal liver. Proliferation and migration assays demonstrated that the 
      increase of SST was related to migration of HOCs, but not their proliferation. 
      RT-PCR and quantitative real-time PCR showed that SSTR4 was preferentially 
      expressed by HOCs. Western blot assay and immunohistochemical staining confirmed 
      the expression of SSTR4 by HOCs. In addition, pretreatment with anti-SSTR4 
      antibody cultures resulted in a dramatic reduction of cell migration as compared 
      to that of control. Lastly, SST stimulated the rearrangement of actin filaments 
      in HOCs, while HOCs treated with anti-SSTR4 antibody failed to do so. These 
      results suggest a positive role for SST in the migration of HOCs, and that this 
      effect is mediated through SSTR4.
FAU - Jung, Youngmi
AU  - Jung Y
AD  - Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, 
      University of Florida, Gainesville, FL 32610-0275, USA.
FAU - Oh, Seh-Hoon
AU  - Oh SH
FAU - Zheng, Donghang
AU  - Zheng D
FAU - Shupe, Thomas D
AU  - Shupe TD
FAU - Witek, Rafal P
AU  - Witek RP
FAU - Petersen, Bryon E
AU  - Petersen BE
LA  - eng
GR  - DK058614/DK/NIDDK NIH HHS/United States
GR  - DK065096/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antibodies)
RN  - 0 (Carcinogens)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Somatostatin)
RN  - 0 (somatostatin receptor subtype-4)
RN  - 51110-01-1 (Somatostatin)
RN  - 9M98QLJ2DL (2-Acetylaminofluorene)
SB  - IM
MH  - 2-Acetylaminofluorene/pharmacology
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Carcinogens/pharmacology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemotaxis
MH  - Disease Models, Animal
MH  - Hepatectomy
MH  - Hepatocytes/drug effects/*physiology
MH  - Liver/cytology/drug effects
MH  - Male
MH  - Membrane Proteins/immunology/*metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Receptors, Somatostatin/immunology/*metabolism
MH  - Somatostatin/*physiology
EDAT- 2006/03/15 09:00
MHDA- 2006/06/28 09:00
CRDT- 2006/03/15 09:00
PHST- 2006/03/15 09:00 [pubmed]
PHST- 2006/06/28 09:00 [medline]
PHST- 2006/03/15 09:00 [entrez]
AID - S0023-6837(22)03282-2 [pii]
AID - 10.1038/labinvest.3700410 [doi]
PST - ppublish
SO  - Lab Invest. 2006 May;86(5):477-89. doi: 10.1038/labinvest.3700410.