PMID- 16535333
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100625
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 62
IP  - 6
DP  - 1996 Jun
TI  - Direct 5S rRNA Assay for Monitoring Mixed-Culture Bioprocesses.
PG  - 1969-76
AB  - This study demonstrates the efficacy of a direct 5S rRNA assay for the 
      characterization of mixed microbial populations by using as an example the 
      bacteria associated with acidic mining environments. The direct 5S rRNA assay 
      described herein represents a nonselective, direct molecular method for 
      monitoring and characterizing the predominant, metabolically active members of a 
      microbial population. The foundation of the assay is high-resolution denaturing 
      gradient gel electrophoresis (DGGE), which is used to separate 5S rRNA species 
      extracted from collected biomass. Separation is based on the unique migration 
      behavior of each 5S rRNA species during electrophoresis in denaturing gradient 
      gels. With mixtures of RNA extracted from laboratory cultures, the upper 
      practical limit for detection in the current experimental system has been 
      estimated to be greater than 15 different species. With this method, the 
      resolution was demonstrated to be effective at least to the species level. The 
      strength of this approach was demonstrated by the ability to discriminate between 
      Thiobacillus ferrooxidans ATCC 19859 and Thiobacillus thiooxidans ATCC 8085, two 
      very closely related species. Migration patterns for the 5S rRNA from members of 
      the genus Thiobacillus were readily distinguishable from those of the genera 
      Acidiphilium and Leptospirillum. In conclusion, the 5S rRNA assay represents a 
      powerful method by which the structure of a microbial population within acidic 
      environments can be assessed.
FAU - Stoner, D L
AU  - Stoner DL
FAU - Browning, C K
AU  - Browning CK
FAU - Bulmer, D K
AU  - Bulmer DK
FAU - Ward, T E
AU  - Ward TE
FAU - Macdonell, M T
AU  - Macdonell MT
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
PMC - PMC1388871
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
PMCR- 1996/06/01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PHST- 1996/06/01 00:00 [pmc-release]
AID - 10.1128/aem.62.6.1969-1976.1996 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1996 Jun;62(6):1969-76. doi: 
      10.1128/aem.62.6.1969-1976.1996.