PMID- 16536741
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20131121
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 7
IP  - 4
DP  - 2006 Apr
TI  - Extracellular ATP stimulates exocytosis via localized Ca(2+) release from acidic 
      stores in rat pancreatic beta cells.
PG  - 429-39
AB  - Three different methods, membrane capacitance (C(m)) measurement, amperometry and 
      FM dye labeling were used to investigate the role of extracellular ATP in insulin 
      secretion from rat pancreatic beta cells. We found that extracellular application 
      of ATP mobilized intracellular Ca(2+) stores and synchronously triggered vigorous 
      exocytosis. No influence of ATP on the readily releasable pool of vesicles was 
      observed, which argues against a direct modulation of the secretory machinery at 
      a level downstream of Ca(2+) elevation. The stimulatory effects of ATP were 
      greatly reduced by intracellular perfusion of BAPTA but not EGTA, suggesting a 
      close spatial association of fusion sites with intracellular Ca(2+) releasing 
      sites. ATP-induced Ca(2+) transients and exocytosis were not blocked by 
      thapsigargin (TG), by a ryanodine receptor antagonist or by dissipation of pH in 
      acidic stores by monensin alone, but they were greatly attenuated by IP(3) 
      receptor inhibition as well as ionomycin plus monensin, suggesting involvement of 
      IP(3)-sensitive acidic Ca(2+) stores. Taken together, our data suggest that 
      extracellular ATP triggers exocytosis by mobilizing spatially limited acidic 
      Ca(2+) stores through IP(3) receptors. This mechanism may explain how insulin 
      secretion from the pancreas is coordinated through diffusible ATP that is 
      co-released with insulin.
FAU - Xie, Li
AU  - Xie L
AD  - Joint Laboratory of Institute of Biophysics and Huazhong University of Science 
      and Technology, National Key Laboratory of Biomacromolecules, Institute of 
      Biophysics, Chinese Academy of Science, Beijing 100101, China.
FAU - Zhang, Ming
AU  - Zhang M
FAU - Zhou, Wei
AU  - Zhou W
FAU - Wu, Zhengxing
AU  - Wu Z
FAU - Ding, Jiuping
AU  - Ding J
FAU - Chen, Liangyi
AU  - Chen L
FAU - Xu, Tao
AU  - Xu T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Calcium Channels)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (FM1 43)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 0 (Insulin)
RN  - 0 (Muscle Relaxants, Central)
RN  - 0 (Pyridinium Compounds)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Ryanodine Receptor Calcium Release Channel)
RN  - 3G6A5W338E (Caffeine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - F64QU97QCR (Dantrolene)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Caffeine/metabolism
MH  - Calcium/*metabolism
MH  - Calcium Channels/metabolism
MH  - Cells, Cultured
MH  - Dantrolene/metabolism
MH  - Electrophysiology
MH  - Enzyme Inhibitors/metabolism
MH  - Exocytosis/*physiology
MH  - Fluorescent Dyes/metabolism
MH  - *Hydrogen-Ion Concentration
MH  - Inositol 1,4,5-Trisphosphate Receptors
MH  - Insulin/*metabolism
MH  - Insulin-Secreting Cells/cytology/*metabolism
MH  - Male
MH  - Muscle Relaxants, Central/metabolism
MH  - Pyridinium Compounds/metabolism
MH  - Quaternary Ammonium Compounds/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Receptors, Purinergic P2/metabolism
MH  - Ryanodine Receptor Calcium Release Channel/metabolism
MH  - Thapsigargin/metabolism
EDAT- 2006/03/16 09:00
MHDA- 2006/07/27 09:00
CRDT- 2006/03/16 09:00
PHST- 2006/03/16 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/03/16 09:00 [entrez]
AID - TRA401 [pii]
AID - 10.1111/j.1600-0854.2006.00401.x [doi]
PST - ppublish
SO  - Traffic. 2006 Apr;7(4):429-39. doi: 10.1111/j.1600-0854.2006.00401.x.