PMID- 16536980
OWN - NLM
STAT- MEDLINE
DCOM- 20061020
LR  - 20150313
VI  - 25
IP  - 3
DP  - 2006 Mar
TI  - [Effect of brain-derived neurotrophic factor on in vitro metastasis of 
      hepatocellular carcinoma cell line HepG2].
PG  - 287-91
AB  - BACKGROUND & OBJECTIVE: Metastasis is one of the important reasons for treatment 
      failure of hepatocellular carcinoma (HCC). It is related to biologic behaviors of 
      HCC, including proliferation, migration, and invasion. Recently, researchers have 
      realized that brain-derived neurotrophic factor (BDNF) is a functional protein 
      that exists in HCC tissue, and closely relates to the development of HCC, but the 
      exact mechanism is still unknown. This study was to explore the effect of BDNF on 
      in vitro metastasis of HCC cell line HepG2, and investigate its mechanism. 
      METHODS: The effect of BDNF on the proliferation of HepG2 cells was examined by 
      MTT assay. The effect of BDNF on the metastasis of HepG2 cells was evaluated by 
      tumor cell migration and invasion assays. The expression of TrkB, a specific 
      receptor of BDNF, in HepG2 cells was detected by reverse transcription-polymerase 
      chain reaction (RT-PCR) and Western blot. RESULTS: BDNF efficiently stimulated 
      the proliferation of HepG2 cells in a dose-dependent manner under the 
      concentration of 100 ng/ml. The migration index was significantly higher in the 
      cells treated with 50 or 100 ng/ml BDNF than in control cells (167 and 203 vs. 
      100, P<0.05). Cell number on the down-side of transwell membrane was 
      significantly higher in the cells treated with 50 or 100 ng/ml BDNF than in 
      control cells (167+/-38 and 215+/-51 vs. 113+/-22, P<0.05). The expression of 
      TrkB was higher in HepG2 than in normal liver cell line L-02. CONCLUSIONS: BDNF 
      can enhance the proliferation, migration, and invasion abilities of HCC cells. It 
      is a novel cytokine which induces metastasis of HCC.
FAU - Xiao, Zhen-Yu
AU  - Xiao ZY
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, P. R. China. 
      xiaozhenyu58@163.com
FAU - Wang, Ya-Dan
AU  - Wang YD
FAU - Chen, Xiao-Ping
AU  - Chen XP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Carcinoma, Hepatocellular/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Liver Neoplasms/metabolism/*pathology
MH  - *Neoplasm Invasiveness
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptor, trkB/*biosynthesis/genetics
EDAT- 2006/03/16 09:00
MHDA- 2006/10/21 09:00
CRDT- 2006/03/16 09:00
PHST- 2006/03/16 09:00 [pubmed]
PHST- 2006/10/21 09:00 [medline]
PHST- 2006/03/16 09:00 [entrez]
AID - 1000467X2006030287 [pii]
PST - ppublish
SO  - Ai Zheng. 2006 Mar;25(3):287-91.