PMID- 16537615
OWN - NLM
STAT- MEDLINE
DCOM- 20060413
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 7
DP  - 2006 Apr
TI  - Measles virus targets DC-SIGN to enhance dendritic cell infection.
PG  - 3477-86
AB  - Dendritic cells (DCs) are involved in the pathogenesis of measles virus (MV) 
      infection by inducing immune suppression and possibly spreading the virus from 
      the respiratory tract to lymphatic tissues. It is becoming evident that DC 
      function can be modulated by the involvement of different receptors in pathogen 
      interaction. Therefore, we have investigated the relative contributions of 
      different MV-specific receptors on DCs to MV uptake into and infection of these 
      cells. DCs express the MV receptors CD46 and CD150, and we demonstrate that the 
      C-type lectin DC-specific intercellular adhesion molecule 3-grabbing nonintegrin 
      (DC-SIGN) is a novel receptor for laboratory-adapted and wild-type MV strains. 
      The ligands for DC-SIGN are both MV glycoproteins F and H. In contrast to CD46 
      and CD150, DC-SIGN does not support MV entry, since DC-SIGN does not confer 
      susceptibility when stably expressed in CHO cells. However, DC-SIGN is important 
      for the infection of immature DCs with MV, since both attachment and infection of 
      immature DCs with MV are blocked in the presence of DC-SIGN inhibitors. Our data 
      demonstrate that DC-SIGN is crucial as an attachment receptor to enhance 
      CD46/CD150-mediated infection of DCs in cis. Moreover, MV might not only target 
      DC-SIGN to infect DCs but may also use DC-SIGN for viral transmission and immune 
      suppression.
FAU - de Witte, Lot
AU  - de Witte L
AD  - Department of Molecular Cell Biology and Immunology, V U University Medical 
      Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.
FAU - Abt, Marion
AU  - Abt M
FAU - Schneider-Schaulies, Sibylle
AU  - Schneider-Schaulies S
FAU - van Kooyk, Yvette
AU  - van Kooyk Y
FAU - Geijtenbeek, Teunis B H
AU  - Geijtenbeek TB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Ligands)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Viral Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - CHO Cells
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Cricetinae
MH  - Dendritic Cells/cytology/immunology/metabolism/*virology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes
MH  - Green Fluorescent Proteins/metabolism
MH  - Humans
MH  - K562 Cells
MH  - Lectins, C-Type/genetics/*metabolism
MH  - Ligands
MH  - Measles/*virology
MH  - Measles virus/genetics/*growth & development/immunology
MH  - Membrane Cofactor Protein/metabolism
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - Viral Fusion Proteins/metabolism
PMC - PMC1440360
EDAT- 2006/03/16 09:00
MHDA- 2006/04/14 09:00
PMCR- 2006/08/01
CRDT- 2006/03/16 09:00
PHST- 2006/03/16 09:00 [pubmed]
PHST- 2006/04/14 09:00 [medline]
PHST- 2006/03/16 09:00 [entrez]
PHST- 2006/08/01 00:00 [pmc-release]
AID - 80/7/3477 [pii]
AID - 1654-05 [pii]
AID - 10.1128/JVI.80.7.3477-3486.2006 [doi]
PST - ppublish
SO  - J Virol. 2006 Apr;80(7):3477-86. doi: 10.1128/JVI.80.7.3477-3486.2006.