PMID- 16537632
OWN - NLM
STAT- MEDLINE
DCOM- 20060413
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 7
DP  - 2006 Apr
TI  - Infection and persistence of rhesus monkey rhadinovirus in immortalized B-cell 
      lines.
PG  - 3644-9
AB  - Similar to its close relative human herpesvirus 8, rhesus monkey rhadinovirus 
      (RRV) persists predominantly in B cells of its natural host. Rhesus monkey B-cell 
      lines immortalized by the Epstein-Barr-related virus from rhesus monkeys (rhEBV) 
      were used as targets for infection by RRV. These cultured B cells were 
      susceptible to infection by RRV and continued to produce low titers of RRV for 
      months of continuous culture. Infection by RRV did not detectably alter the 
      growth rates of these B-cell lines when it was measured at standard or reduced 
      serum concentrations. Depending on the cell line, 5 to 40% of the B cells stained 
      positive for the RRV genome by fluorescence in situ hybridization (FISH). Most 
      RRV-positive cells showed a fine punctate nuclear staining pattern consistent 
      with latent infection, while a small minority of cells (0.2 to 1%) contained 
      large, intensely staining nuclear foci consistent with productive, replicative 
      infection. Greater than 90% of the cells were rhEBV genome positive in a pattern 
      consistent with latent infection, and again only a small minority of cells showed 
      a productive, replicative staining pattern. Dual, two-color FISH staining 
      revealed coinfection of numerous cells with both RRV and rhEBV, but productive 
      replication of RRV and rhEBV was always observed in separate cells, never in the 
      same cell. Thus, productive replication of RRV is unlinked to that of rhEBV; 
      factors that influence activation to productive replication act separately on RRV 
      and rhEBV, even within the same cell. The percentage of B cells expressing green 
      fluorescent protein (GFP) early after infection with a recombinant RRV containing 
      a GFP reporter gene was dose dependent and at a low multiplicity of infection 
      increased progressively over time until 14 to 17 days after infection. These 
      results establish a naturalistic cell culture system for the study of infection 
      and persistence by RRV in rhesus monkey B cells.
FAU - Bilello, John P
AU  - Bilello JP
AD  - New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, 
      Southborough, MA 01772-9102, USA.
FAU - Lang, Sabine M
AU  - Lang SM
FAU - Wang, Fred
AU  - Wang F
FAU - Aster, Jon C
AU  - Aster JC
FAU - Desrosiers, Ronald C
AU  - Desrosiers RC
LA  - eng
GR  - R01 AI063928/AI/NIAID NIH HHS/United States
GR  - P01 DE014388/DE/NIDCR NIH HHS/United States
GR  - RR00168/RR/NCRR NIH HHS/United States
GR  - P51 RR000168/RR/NCRR NIH HHS/United States
GR  - K26 RR000168/RR/NCRR NIH HHS/United States
GR  - 5T32AI0724522/AI/NIAID NIH HHS/United States
GR  - 1P01DE14388/DE/NIDCR NIH HHS/United States
GR  - 1R01AI063928/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Recombinant Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*virology
MH  - Blotting, Western
MH  - Cell Culture Techniques
MH  - Cell Line, Transformed
MH  - Cell Nucleus/metabolism/virology
MH  - Cell Transformation, Viral
MH  - Fibroblasts/metabolism/virology
MH  - Genes, Reporter
MH  - Genome, Viral
MH  - Green Fluorescent Proteins/metabolism
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 4, Human/isolation & purification/pathogenicity/physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Macaca mulatta/*virology
MH  - Recombinant Proteins/metabolism
MH  - Rhadinovirus/genetics/isolation & purification/pathogenicity/*physiology
MH  - Time Factors
MH  - Tumor Virus Infections/*virology
MH  - Virus Replication
PMC - PMC1440374
EDAT- 2006/03/16 09:00
MHDA- 2006/04/14 09:00
PMCR- 2006/08/01
CRDT- 2006/03/16 09:00
PHST- 2006/03/16 09:00 [pubmed]
PHST- 2006/04/14 09:00 [medline]
PHST- 2006/03/16 09:00 [entrez]
PHST- 2006/08/01 00:00 [pmc-release]
AID - 80/7/3644 [pii]
AID - 1415-05 [pii]
AID - 10.1128/JVI.80.7.3644-3649.2006 [doi]
PST - ppublish
SO  - J Virol. 2006 Apr;80(7):3644-9. doi: 10.1128/JVI.80.7.3644-3649.2006.