PMID- 1653890
OWN - NLM
STAT- MEDLINE
DCOM- 19911016
LR  - 20081121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 5
IP  - 3
DP  - 1991 Mar
TI  - Evidence for a protein-trafficking gene that rescues the defective 
      glucocorticoid-regulated transport and Golgi retention of mouse mammary tumor 
      virus glycoproteins in a rat hepatoma cell-sorting variant.
PG  - 336-46
AB  - Glucocorticoids regulate the trafficking of cell surface mouse mammary tumor 
      virus (MMTV) glycoproteins in the virus-infected rat hepatoma cell line M1.54. 
      The CR4 rat hepatoma sorting variant, which is derived from M1.54 cells by 
      immunoselection, is uniquely defective in the glucocorticoid-regulated transport 
      of MMTV glycoproteins. Indirect immunofluorescence of fixed permeabilized cells 
      and subcellular fractionation of isolated microsomes revealed that variant CR4 
      cells retain the MMTV glycoproteins in Golgi-like membranes after glucocorticoid 
      treatment. The variant CR4 phenotype can be complemented by interspecies cell 
      fusions with human HepG2 hepatoma cells and by DNA rescue with genomic fragments 
      isolated from either human or rat hepatoma cells. Transfected wild-type genomic 
      fragments rescue the sorting defect in CR4 at a frequency consistant with a 
      single genetic locus, whereas homologous transfection with CR4 genomic DNA has no 
      effect. Thus, complementation of a rat hepatoma cell-sorting variant supports the 
      existence of a novel protein-trafficking activity encoded by the human or rat 
      genomes that acts in trans in the Golgi to selectively mediate the sorting of 
      cell surface MMTV glycoproteins in glucocorticoid-treated cells.
FAU - Bravo, D A
AU  - Bravo DA
AD  - Department of Molecular and Cell Biology, University of California, Berkeley 
      94720.
FAU - Goodman, L J
AU  - Goodman LJ
FAU - Firestone, G L
AU  - Firestone GL
LA  - eng
GR  - CA-09041/CA/NCI NIH HHS/United States
GR  - DK-42799/DK/NIDDK NIH HHS/United States
GR  - GM-10274/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (Glucocorticoids)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antigens, Viral, Tumor/*genetics
MH  - Biological Transport
MH  - Carcinoma, Hepatocellular/*genetics/pathology
MH  - DNA/metabolism
MH  - Genetic Complementation Test
MH  - Genetic Variation
MH  - Glucocorticoids/*pharmacology
MH  - Glycoproteins/*genetics
MH  - Golgi Apparatus/drug effects/*metabolism
MH  - Humans
MH  - *Mammary Tumor Virus, Mouse/analysis
MH  - Phenotype
MH  - Rats
MH  - Tumor Cells, Cultured
MH  - Viral Envelope Proteins/*genetics
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - 10.1210/mend-5-3-336 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1991 Mar;5(3):336-46. doi: 10.1210/mend-5-3-336.