PMID- 16539650
OWN - NLM
STAT- MEDLINE
DCOM- 20060608
LR  - 20161128
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 97
IP  - 2
DP  - 2006 Apr
TI  - D-Aspartate as a putative cell-cell signaling molecule in the Aplysia californica 
      central nervous system.
PG  - 595-606
AB  - The content, synthesis and transport of D-aspartate (D-Asp) in the CNS of Aplysia 
      californica is investigated using capillary electrophoresis (CE) with both 
      laser-induced fluorescence and radionuclide detection. Millimolar concentrations 
      of D-Asp are found in various regions of the CNS. In the cerebral ganglion, three 
      adjacent neuronal clusters have reproducibly different D-Asp levels; for example, 
      in the F- and C-clusters, up to 85% of the free Asp is present in the D-form. 
      Heterogeneous distribution of D-Asp is also found in the individual identified 
      neurons tested, including the optical ganglion top-layer neurons, metacerebral 
      cells, R2 neurons, and F-, C- and G-cluster neurons. The F-cluster neurons have 
      the highest percentage of D-Asp (approximately 58% of the total Asp), whereas the 
      lowest value of approximately 8% is found in R2 neurons. In pulse-chase 
      experiments with radiolabeled D-Asp, followed by CE with radionuclide detection, 
      the synthesis of D-Asp from L-aspartate (L-Asp) is confirmed. Is D-Asp in the 
      soma, or is it transported to distantly located release sites? D-Asp is clearly 
      detected in the major nerves of A. californica, including the pleuroabdominal and 
      cerebrobuccal connectives and the anterior tentacular nerves, suggesting it is 
      transported long distances. In addition, both D-Asp and L-Asp are transported in 
      the pleuroabdominal connectives in a colchicine-dependent manner, whereas several 
      other amino acids are not. Finally, d-Asp produces electrophysiological effects 
      similar to those induced by L-Asp. These data are consistent with an active role 
      for D-Asp in cell-to-cell communication.
FAU - Miao, Hai
AU  - Miao H
AD  - Department of Chemistry and the Beckman Institute, University of Illinois at 
      Urbana-Champaign, 61801, USA.
FAU - Rubakhin, Stanislav S
AU  - Rubakhin SS
FAU - Scanlan, Cory R
AU  - Scanlan CR
FAU - Wang, Liping
AU  - Wang L
FAU - Sweedler, Jonathan V
AU  - Sweedler JV
LA  - eng
GR  - P30 DA018310/DA/NIDA NIH HHS/United States
GR  - R33 DK070285/DK/NIDDK NIH HHS/United States
GR  - DK070285/DK/NIDDK NIH HHS/United States
GR  - R01 NS031609/NS/NINDS NIH HHS/United States
GR  - P30 DA018301/DA/NIDA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060315
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Animals
MH  - Aplysia/anatomy & histology/drug effects/*metabolism
MH  - Aspartic Acid/metabolism/pharmacology
MH  - Biological Transport
MH  - Central Nervous System/*cytology/metabolism
MH  - Colchicine/pharmacology
MH  - D-Aspartic Acid/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Electrophoresis, Capillary/methods
MH  - Electrophysiology/methods
MH  - Fluorescence
MH  - Membrane Potentials/drug effects
MH  - Neurons/classification/diagnostic imaging/drug effects/*physiology
MH  - Radionuclide Imaging/methods
MH  - Signal Transduction/*physiology
EDAT- 2006/03/17 09:00
MHDA- 2006/06/09 09:00
CRDT- 2006/03/17 09:00
PHST- 2006/03/17 09:00 [pubmed]
PHST- 2006/06/09 09:00 [medline]
PHST- 2006/03/17 09:00 [entrez]
AID - JNC3791 [pii]
AID - 10.1111/j.1471-4159.2006.03791.x [doi]
PST - ppublish
SO  - J Neurochem. 2006 Apr;97(2):595-606. doi: 10.1111/j.1471-4159.2006.03791.x. Epub 
      2006 Mar 15.