PMID- 16540266
OWN - NLM
STAT- MEDLINE
DCOM- 20070816
LR  - 20060529
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 115
IP  - 1-3
DP  - 2006 Jun 15
TI  - Mycoplasma hyorhinis in Taiwan: diagnosis and isolation of swine pneumonia 
      pathogen.
PG  - 111-6
AB  - This study attempted to determine whether one multiplex polymerase chain reaction 
      (PCR) is an effective adjunct method for diagnosing Mycoplasma hyopneumoniae and 
      Mycoplasma hyorhinis infection, and whether M. hyorhinis should be considered as 
      an enzootic pneumonia or porcine respiratory disease complex pathogen in Taiwan. 
      To our knowledge, this study is the first to isolate and identify M. hyorhinis as 
      a porcine pathogen in Taiwan. A novel isolation method and a multiplex PCR test 
      were applied to detect and isolate M. hyorhinis. The correlation of M. hyorhinis 
      with swine pneumonia was also examined using a challenge test. Based on weight, 
      18 pigs were assigned to three groups and housed throughout the study in a 
      specific-pathogen-free (SPF) facility and provided with aseptic feed and water. 
      Groups 1 (n=6) and 2 (n=6) were challenged with 5mL M. hyorhinis culture via 
      tracheal intubation on day 1. The M. hyorhinis strains ATIT-1, -3, and-7 were 
      used to infect group 1 and the strain ATCC 27717 was used for group 2. Culture 
      medium was replaced by phosphate-buffered saline in group 3 (n=6). All pigs were 
      slaughtered on day 28, and their lungs were removed for examination of lesions. 
      Of the six pigs in group 1 challenged with wild-type strains, two had typical 
      mycoplasma pneumonia lesions. No gross lung lesions were observed in groups 2 and 
      3. Although further examination is necessary to confirm that wild-type strains 
      can cause pneumonia, it appears that M. hyopneumoniae is no longer the only 
      mycoplasma pathogen implicated in the diagnosis of swine enzootic pneumonia 
      (SEP).
FAU - Lin, J H
AU  - Lin JH
AD  - Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli, 
      Taiwan, ROC.
FAU - Chen, S P
AU  - Chen SP
FAU - Yeh, K S
AU  - Yeh KS
FAU - Weng, C N
AU  - Weng CN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060315
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - DNA, Bacterial/chemistry/isolation & purification
MH  - Diagnosis, Differential
MH  - Lung/microbiology/pathology
MH  - Mycoplasma/classification/genetics/*isolation & purification/*pathogenicity
MH  - Mycoplasma hyopneumoniae/isolation & purification/pathogenicity
MH  - Pneumonia of Swine, Mycoplasmal/diagnosis/microbiology
MH  - Pneumonia, Mycoplasma/diagnosis/epidemiology/microbiology/*veterinary
MH  - Polymerase Chain Reaction/methods/*veterinary
MH  - Random Allocation
MH  - Sensitivity and Specificity
MH  - Specific Pathogen-Free Organisms
MH  - Swine
MH  - Swine Diseases/*diagnosis/epidemiology/microbiology
MH  - Taiwan/epidemiology
EDAT- 2006/03/17 09:00
MHDA- 2007/08/19 09:00
CRDT- 2006/03/17 09:00
PHST- 2004/09/08 00:00 [received]
PHST- 2006/02/06 00:00 [revised]
PHST- 2006/02/06 00:00 [accepted]
PHST- 2006/03/17 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2006/03/17 09:00 [entrez]
AID - S0378-1135(06)00051-4 [pii]
AID - 10.1016/j.vetmic.2006.02.004 [doi]
PST - ppublish
SO  - Vet Microbiol. 2006 Jun 15;115(1-3):111-6. doi: 10.1016/j.vetmic.2006.02.004. 
      Epub 2006 Mar 15.