PMID- 16541297
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20061009
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 128
IP  - 1-3
DP  - 2006 Sep-Nov
TI  - Gene mapping of 28S and 5S rDNA sites in the spined loach Cobitis taenia (Pisces, 
      Cobitidae) from a diploid population and a diploid-tetraploid population.
PG  - 71-9
AB  - We compare the chromosomal 28S and 5S rDNA patterns of the spined loach C. taenia 
      (2n = 48) from an exclusively diploid population and from a diploid-polyploid 
      population using 28S and 5S rDNA probe preparation and labelling, and 
      fluorescence in situ hybridization (FISH). The 5S rDNA was located in two to 
      three chromosome pairs, and separated from the 28S loci for the males and one 
      female (F1) from the diploid population. Loaches from a diploid-polyploid 
      population, and one female (F2) from the diploid population were characterized by 
      at least one chromosome pair with 5S and 28S overlapping signals. The fishes 
      differed mainly in their number of 28S rDNA loci, located on 3-6 chromosomes. All 
      individuals from both populations were characterized by one acrocentric 
      chromosome bearing a 28S rDNA signal on the telomeres of its long arm. The number 
      of major ribosomal DNA in the karyotype of C. taenia by FISH was always higher 
      than the number of Ag-NORs. Our data confirm the extensive polymorphism of NORs 
      in both populations, as already has been observed in closely related Cobitis 
      species, and less polymorphic 5S rDNA pattern. However, this preliminary result 
      highlights the need for a wider scale study.
FAU - Boron, Alicja
AU  - Boron A
AD  - Department of Zoology, University of Warmia and Mazury, M. Oczapowski 5 St., 
      10-718, Olsztyn, Poland. alibo@uwm.edu.pl
FAU - Ozouf-Costaz, Catherine
AU  - Ozouf-Costaz C
FAU - Coutanceau, Jean-Pierre
AU  - Coutanceau JP
FAU - Woroniecka, Katarzyna
AU  - Woroniecka K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 28S)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Cypriniformes/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Diploidy
MH  - Female
MH  - Genetics, Population
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nucleolus Organizer Region
MH  - Poland
MH  - Polyploidy
MH  - RNA, Ribosomal, 28S/genetics
MH  - RNA, Ribosomal, 5S/genetics
EDAT- 2006/03/17 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/03/17 09:00
PHST- 2005/05/30 00:00 [received]
PHST- 2005/11/25 00:00 [accepted]
PHST- 2006/03/17 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/03/17 09:00 [entrez]
AID - 10.1007/s10709-005-5536-8 [doi]
PST - ppublish
SO  - Genetica. 2006 Sep-Nov;128(1-3):71-9. doi: 10.1007/s10709-005-5536-8.