PMID- 16542405
OWN - NLM
STAT- MEDLINE
DCOM- 20060620
LR  - 20131121
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 56
IP  - 1
DP  - 2006 Apr
TI  - Effects of naphthalene on microbial community composition in the Delaware 
      estuary.
PG  - 55-63
AB  - The effects of naphthalene on microbial communities in the bottom boundary layer 
      of the Delaware Bay estuary were investigated in microcosms using denaturing 
      gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) 
      with oligonucleotide probes. Three days after the addition of naphthalene, rates 
      of bacterial production and naphthalene mineralization were higher than in 
      no-addition controls and than in cases where glucose was added. Analyses using 
      both DGGE and FISH indicated that the bacterial community changed in response to 
      the addition of naphthalene. FISH data indicated that a few major phylogenetic 
      groups increased in response to the glucose addition and especially to the 
      naphthalene addition. DGGE also demonstrated differences in community composition 
      among treatments, with four phylotypes being unique to naphthalene-amended 
      treatments and three of these having 16S rRNA genes similar to known hydrocarbon 
      degraders. The bacterial community in the naphthalene-amended treatment was 
      distinct from the communities in the glucose-amended treatment and in the 
      no-addition control. These data suggest that polycyclic aromatic hydrocarbons may 
      have large effects on microbial community structure in estuaries and probably on 
      microbially mediated biogeochemical processes.
FAU - Castle, Dawn M
AU  - Castle DM
AD  - College of Marine Studies, University of Delaware, Lewes, DE 19958, USA.
FAU - Montgomery, Michael T
AU  - Montgomery MT
FAU - Kirchman, David L
AU  - Kirchman DL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (Naphthalenes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Water Pollutants, Chemical)
RN  - 2166IN72UN (naphthalene)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acinetobacter/drug effects/genetics/metabolism
MH  - Bacteria/*drug effects/genetics/metabolism
MH  - Base Sequence
MH  - DNA, Bacterial/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glucose/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Naphthalenes/metabolism/*pharmacology
MH  - Phylogeny
MH  - Proteobacteria/drug effects/genetics/metabolism
MH  - Pseudomonas/drug effects/genetics/metabolism
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Seawater
MH  - Sequence Analysis, DNA
MH  - *Water Microbiology
MH  - Water Pollutants, Chemical/metabolism/*pharmacology
EDAT- 2006/03/18 09:00
MHDA- 2006/06/21 09:00
CRDT- 2006/03/18 09:00
PHST- 2006/03/18 09:00 [pubmed]
PHST- 2006/06/21 09:00 [medline]
PHST- 2006/03/18 09:00 [entrez]
AID - FEM062 [pii]
AID - 10.1111/j.1574-6941.2006.00062.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2006 Apr;56(1):55-63. doi: 10.1111/j.1574-6941.2006.00062.x.