PMID- 16544187
OWN - NLM
STAT- MEDLINE
DCOM- 20060803
LR  - 20220316
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 14
IP  - 2
DP  - 2006
TI  - Chromosomal integrity maintained in five human embryonic stem cell lines after 
      prolonged in vitro culture.
PG  - 131-7
AB  - There have been recent reports of human embryonic stem cell (hESC) lines 
      developing chromosomal aberrations after long-term culture, indicating an 
      unstable genomic status due to the in vitro milieu. This raises concern, since it 
      would limit their use in therapeutics. In this study the chromosomal status of 
      five well-characterized hESC lines, SA002, SA002.5, AS034.1.1, SA121 and SA461, 
      was monitored during long-term in vitro culture. The criteria of defined hESCs 
      were met by all of the five hESC lines (four diploid and one trisomic for 
      chromosome 13). The genomes were screened for chromosomal aberrations and 
      rearrangements using comparative genomic hybridization (CGH), interphase 
      fluorescence in situ hybridization (FISH) and traditional karyotyping on several 
      occasions while in culture. The genomic integrity was shown to be maintained 
      after repeated freeze-thaw procedures and continuous culture in vitro for up to 
      22 months (148 passages). We discuss the most common de novo chromosomal 
      aberrations reported in hESCs, as well as their possible origin.
FAU - Caisander, Gunilla
AU  - Caisander G
AD  - Cellartis AB, Arvid Wallgrens Backe 20, SE-413 46, Goteborg, Sweden.
FAU - Park, Hannah
AU  - Park H
FAU - Frej, Katarina
AU  - Frej K
FAU - Lindqvist, Jenny
AU  - Lindqvist J
FAU - Bergh, Christina
AU  - Bergh C
FAU - Lundin, Kersti
AU  - Lundin K
FAU - Hanson, Charles
AU  - Hanson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060317
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - *Cell Culture Techniques
MH  - Cell Line
MH  - Cell Proliferation
MH  - *Chromosomal Instability
MH  - Chromosome Aberrations
MH  - Chromosomes, Human
MH  - Embryo, Mammalian/*cytology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Nucleic Acid Hybridization
MH  - Retrospective Studies
MH  - Stem Cells/*metabolism
EDAT- 2006/03/18 09:00
MHDA- 2006/08/04 09:00
CRDT- 2006/03/18 09:00
PHST- 2005/10/08 00:00 [received]
PHST- 2005/12/09 00:00 [accepted]
PHST- 2005/12/09 00:00 [revised]
PHST- 2006/03/18 09:00 [pubmed]
PHST- 2006/08/04 09:00 [medline]
PHST- 2006/03/18 09:00 [entrez]
AID - 10.1007/s10577-006-1019-8 [doi]
PST - ppublish
SO  - Chromosome Res. 2006;14(2):131-7. doi: 10.1007/s10577-006-1019-8. Epub 2006 Mar 
      17.