PMID- 1654446
OWN - NLM
STAT- MEDLINE
DCOM- 19911022
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 65
IP  - 10
DP  - 1991 Oct
TI  - Identification of a transactivating function mapping to the putative 
      immediate-early locus of human herpesvirus 6.
PG  - 5381-90
AB  - Sequencing studies have indicated that the unique component of the human 
      herpesvirus 6 (HHV-6) genome and the unique long segment of the human 
      cytomegalovirus genome are genetically colinear. Of particular interest is the 
      identification of a region of local CpG dinucleotide suppression in the genome of 
      HHV-6, a feature conserved in the genomes of human cytomegalovirus, murine 
      cytomegalovirus, and simian cytomegalovirus, and a characteristic of the major 
      immediate-early loci of these viruses. Adjacent to this region in HHV-6 are 
      approximately 30 copies of a 103- to 108-bp sequence element, which contains 
      consensus binding sites for the transcription factors AP2 and NF kappa B, in 
      addition to a single KpnI recognition site. Together, these KpnI repeat units may 
      compose an immediate-early enhancer, analogous to those found in the 
      cytomegaloviruses. We present the sequence of this region of HHV-6 and 
      demonstrate that a transactivating function is encoded by this region. We have 
      used polymerase chain reaction to synthesize fragments containing open reading 
      frames and 5' sequences with or without the upstream KpnI repeat units. Effector 
      plasmids containing these HHV-6 coding and 5' sequences were able to effect 
      activation of heterologous promoter-chloramphenicol acetyltransferase (CAT) 
      constructs, including adenovirus E3-CAT and E4-CAT, human T-cell lymphotropic 
      virus type I long terminal repeat (LTR)-CAT, and human immunodeficiency virus 
      LTR-CAT, in cotransfection experiments in Vero cells and peripheral blood 
      lymphocytes. Furthermore, we have identified the major open reading frame (RF4; 
      2.3 kb) as being essential for activation, and we have shown that the NF kappa B, 
      SP1, and TATA box motifs in the human immunodeficiency virus LTR are all required 
      for full induction of the promoter by the HHV-6-encoded transactivator.
FAU - Martin, M E
AU  - Martin ME
AD  - Division of Virology, National Institute for Medical Research, London, United 
      Kingdom.
FAU - Nicholas, J
AU  - Nicholas J
FAU - Thomson, B J
AU  - Thomson BJ
FAU - Newman, C
AU  - Newman C
FAU - Honess, R W
AU  - Honess RW
LA  - eng
SI  - GENBANK/M63043
SI  - GENBANK/M63044
SI  - GENBANK/M63045
SI  - GENBANK/M63046
SI  - GENBANK/M63047
SI  - GENBANK/M63048
SI  - GENBANK/M73681
SI  - GENBANK/S56129
SI  - GENBANK/S56146
SI  - GENBANK/S56349
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - 0 (Oligonucleotide Probes)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chloramphenicol O-Acetyltransferase/genetics/metabolism
MH  - Cytomegalovirus/genetics
MH  - DNA, Viral/*genetics/isolation & purification
MH  - Enhancer Elements, Genetic
MH  - *Genes, Viral
MH  - Herpesvirus 6, Human/*genetics/growth & development
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - TATA Box
MH  - *Transcriptional Activation
MH  - Transfection
MH  - Vero Cells
PMC - PMC249019
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
PMCR- 1991/10/01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
PHST- 1991/10/01 00:00 [pmc-release]
AID - 10.1128/JVI.65.10.5381-5390.1991 [doi]
PST - ppublish
SO  - J Virol. 1991 Oct;65(10):5381-90. doi: 10.1128/JVI.65.10.5381-5390.1991.