PMID- 1654497
OWN - NLM
STAT- MEDLINE
DCOM- 19911023
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 40
IP  - 9
DP  - 1991 Sep
TI  - Tumor necrosis factor-alpha, interleukin-1, and interferon alpha stimulate 
      triglyceride synthesis in HepG2 cells.
PG  - 894-8
AB  - Tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), and interferon 
      alpha (IFN-alpha) stimulate hepatic lipogenesis in vivo. We now show that 
      TNF-alpha, IL-1, and IFN-alpha stimulate lipogenesis as measured by the 
      incorporation of 3H-glycerol into triglyceride in cultured HepG2 cells. 
      Incubation of HepG2 cells for approximately 24 hours with TNF or IL-1 was 
      required to see stimulation of lipogenesis, with this effect increasing over the 
      next four days. TNF stimulated lipogenesis by 2.4-fold after 72 hours of 
      incubation, while a 3.5-fold stimulation was seen with IL-1. The half maximal 
      concentration for TNF stimulation of hepatic lipogenesis was 4 ng/mL, while that 
      for IL-1 was 0.3 ng/mL. Cells treated with TNF or IL-1 also showed increased 
      secretion of labeled triglyceride into the media. IFN-alpha stimulated the 
      incorporation of 3H-glycerol into triglyceride by 39% after 72 hour's incubation. 
      In contrast, IFN-gamma had no effect on lipogenesis in HepG2 cells. These data 
      suggest that cytokines can directly stimulate the synthesis of triglycerides in 
      cultured Hep G2 liver cells in vitro.
FAU - Grunfeld, C
AU  - Grunfeld C
AD  - Department of Medicine, University of California, San Francisco.
FAU - Dinarello, C A
AU  - Dinarello CA
FAU - Feingold, K R
AU  - Feingold KR
LA  - eng
GR  - AI15614/AI/NIAID NIH HHS/United States
GR  - DK40990/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Interferon Type I)
RN  - 0 (Interleukin-1)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Carcinoma, Hepatocellular/*metabolism/pathology
MH  - Humans
MH  - Interferon Type I/*pharmacology
MH  - Interleukin-1/*pharmacology
MH  - Liver Neoplasms/*metabolism/pathology
MH  - Triglycerides/*biosynthesis
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 0026-0495(91)90062-2 [pii]
AID - 10.1016/0026-0495(91)90062-2 [doi]
PST - ppublish
SO  - Metabolism. 1991 Sep;40(9):894-8. doi: 10.1016/0026-0495(91)90062-2.