PMID- 16545095
OWN - NLM
STAT- MEDLINE
DCOM- 20060616
LR  - 20191210
IS  - 0300-5127 (Print)
IS  - 0300-5127 (Linking)
VI  - 34
IP  - Pt 2
DP  - 2006 Apr
TI  - Manipulation of dendritic cells for host defence against intracellular 
      infections.
PG  - 283-6
AB  - Dendritic cells (DCs) are an important innate immune cell type which is the 
      bridge between innate and adaptive immunity. Mounting experimental evidence 
      suggests that manipulating DCs represents a powerful means to enhance host 
      defence against intracellular infectious diseases. We have developed several 
      strategies to manipulate DCs either in vivo or in vitro for the purpose of 
      enhancing the effect of vaccination or immunotherapeutics. In vivo delivery of 
      transgene encoding GM-CSF (granulocyte/macrophage colony-stimulating factor), a 
      DC-activating cytokine, increases the number and activation status of DCs at 
      various tissue sites and enhances antimicrobial immune responses in murine 
      models. Co-expression or co-delivery of GM-CSF gene transfer vector with an 
      antimicrobial vaccine enhances microbial antigen-specific T-cell responses and 
      immune protection. Murine bone marrow-derived DCs are being manipulated in vitro 
      and exploited as a vaccine delivery system. Transduction of DCs with a 
      virus-vectored tuberculosis vaccine is a powerful way to activate T-cells in 
      vivo. Such genetically modified DC vaccines can be administered either 
      parenterally or mucosally via the respiratory tract.
FAU - McCormick, S
AU  - McCormick S
AD  - Department of Pathology and Molecular Medicine and Division of Infectious 
      Diseases, Centre for Gene Therapeutics, McMaster University, Hamilton, ON, 
      Canada, L8N 3Z5.
FAU - Santosuosso, M
AU  - Santosuosso M
FAU - Zhang, X Z
AU  - Zhang XZ
FAU - Xing, Z
AU  - Xing Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Soc Trans
JT  - Biochemical Society transactions
JID - 7506897
RN  - 0 (Adjuvants, Immunologic)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adjuvants, Immunologic
MH  - Animals
MH  - Cell Communication
MH  - Cell Proliferation/drug effects
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/immunology/pharmacology
MH  - Infections/*immunology/microbiology/*pathology
MH  - Intracellular Space/*microbiology/pathology
EDAT- 2006/03/21 09:00
MHDA- 2006/06/17 09:00
CRDT- 2006/03/21 09:00
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/06/17 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - BST20060283 [pii]
AID - 10.1042/BST20060283 [doi]
PST - ppublish
SO  - Biochem Soc Trans. 2006 Apr;34(Pt 2):283-6. doi: 10.1042/BST20060283.