PMID- 16545788
OWN - NLM
STAT- MEDLINE
DCOM- 20060915
LR  - 20131121
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 370
IP  - 1-2
DP  - 2006 Aug
TI  - Correlation between a gene polymorphism of tumor necrosis factor-alpha (G/A) and 
      end-stage renal disease: a pilot study from north India.
PG  - 152-7
AB  - BACKGROUND: Patients with chronic kidney disease manifest an inflammatory state 
      in comparison to healthy individuals. Tumor necrosis factor-alpha (TNF-alpha) is 
      a potent pro-inflammatory cytokine involved in initiation and progression of 
      renal injury. We examined the 2-promoter region polymorphism of TNF-alpha gene G 
      to A at -308 and at +488 sites in end-stage renal disease (ESRD) subjects. 
      METHODS: The TNF-alpha -308 G/A and +488 G/A polymorphisms were genotyped in 231 
      patients aged 36.5+/-10, and in 180 matched controls (34.96+/-11.3) by polymerase 
      chain reaction-restriction fragment length polymorphism (PCR-RFLP) and 
      amplification refractory mutation system (ARMS-PCR) method, respectively. 
      RESULTS: The genotypic distribution of TNF-alpha -308 and +488 were significantly 
      different between patients and controls (P<0.001 and P<0.006), respectively. The 
      AA genotype was more frequent in ESRD patients than controls for both the sites 
      (42% vs. 2.8% and 17.3% vs. 2.2%), respectively. The allelic frequency of 
      TNF-alpha A was also higher in cases than in controls for both the sites 
      (P<0.001; OR=2.96; 95% CI=2.228-3.945 and P<0.013; OR=1.422; 95% CI=1.078-1.876). 
      Significant difference was observed for haplotype frequency distribution between 
      ESRD patients and controls and 'A-G#' haplotype showed >9-fold higher risk 
      (OR=9.886, 95% CI=4.408-22.172). The two polymorphisms were in linkage 
      disequilibrium in the control group (D'=0.8047, P<0.001). CONCLUSION: Both the 
      variants of TNF-alpha (-308 and +488) polymorphism had significant association 
      and may thus be a strong predisposing risk factor for ESRD in a cohort of north 
      Indian population. Further, individuals with haplotypes A-G# may be at higher 
      risk for ESRD.
FAU - Manchanda, Parmeet Kaur
AU  - Manchanda PK
AD  - Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, Uttar Pradesh, 
      India.
FAU - Kumar, Anant
AU  - Kumar A
FAU - Kaul, Anupma
AU  - Kaul A
FAU - Mittal, Rama Devi
AU  - Mittal RD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060228
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (TNF protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 12133JR80S (Guanosine)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*genetics
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - Diabetes Mellitus/genetics
MH  - Female
MH  - Genotype
MH  - Glomerulonephritis/genetics
MH  - Guanosine/*genetics
MH  - Humans
MH  - India
MH  - Kidney Failure, Chronic/*genetics
MH  - Male
MH  - Middle Aged
MH  - Nephrosis/etiology/genetics
MH  - Pilot Projects
MH  - Polymorphism, Genetic/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2006/03/21 09:00
MHDA- 2006/09/16 09:00
CRDT- 2006/03/21 09:00
PHST- 2005/12/16 00:00 [received]
PHST- 2006/02/04 00:00 [revised]
PHST- 2006/02/06 00:00 [accepted]
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/09/16 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - S0009-8981(06)00111-2 [pii]
AID - 10.1016/j.cca.2006.02.002 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2006 Aug;370(1-2):152-7. doi: 10.1016/j.cca.2006.02.002. Epub 
      2006 Feb 28.