PMID- 16545883
OWN - NLM
STAT- MEDLINE
DCOM- 20060921
LR  - 20191210
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 117
IP  - 2
DP  - 2006 May
TI  - Stimulation of interferon-beta gene expression by human cytomegalovirus via 
      nuclear factor kappa B and phosphatidylinositol 3-kinase pathway.
PG  - 209-14
AB  - Infection of human foreskin fibroblast (HFF) cells with human cytomegalovirus 
      (HCMV) induces the secretion of soluble factors including interferon (IFN)-beta 
      that stimulates human leukocyte antigen (HLA) class I expression. In this study, 
      the mechanism of IFN-beta induction by HCMV was investigated. In HCMV-infected 
      HFF cells, IFN-beta secretion increased at 6h post infection (h.p.i.). Reverse 
      transcription polymerase chain reaction (RT-PCR) analysis using ultra violet 
      (UV)-inactivated HCMV indicated that viral gene expression is not necessary for 
      the stimulation of IFN-beta. Stimulation of IFN-beta by HCMV infection was not 
      blocked by cycloheximide, an inhibitor of protein synthesis, further suggesting 
      that the expression of HCMV genes is not required for the stimulation of IFN-beta 
      gene transcription. IFN-beta may be produced from virus-infected cells as an 
      inflammatory response and nuclear factor kappa B (NF-kappaB) plays a central role 
      in inflammatory response. HCMV failed to induce the IFN-beta expression, when the 
      virus-infected cells were treated with pyrrolidine dithiocarbamate (PDTC), an 
      inhibitor of NF-kappaB, or LY294002 and wortmannin, inhibitors of 
      phosphatidylinositol 3-kinase (PI3-K). The result suggests that PI3-K and/or 
      NF-kappaB may be related with the induction pathway of IFN-beta by HCMV.
FAU - Lee, Gyu Cheol
AU  - Lee GC
AD  - Division of Life Sciences, College of Natural Sciences, and Biotechnology 
      Research Institute, Chungbuk National University, 12 Gaeshindong, Cheongju, 
      Chungbuk 361-763, South Korea. chlee@chungbuk.ac.kr
FAU - Yi, Hyun Ah
AU  - Yi HA
FAU - Lee, Chan Hee
AU  - Lee CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060320
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Androstadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (NF-kappa B)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Pyrrolidines)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Thiocarbamates)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
RN  - 77238-31-4 (Interferon-beta)
RN  - 98600C0908 (Cycloheximide)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Androstadienes/pharmacology
MH  - Cell Line
MH  - Cycloheximide/pharmacology
MH  - Cytomegalovirus/*immunology
MH  - Enzyme Inhibitors/pharmacology
MH  - Fibroblasts/virology
MH  - *Gene Expression
MH  - Humans
MH  - Interferon-beta/*biosynthesis/*genetics
MH  - NF-kappa B/antagonists & inhibitors/*physiology
MH  - Phosphatidylinositol 3-Kinases/*physiology
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Pyrrolidines/pharmacology
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thiocarbamates/pharmacology
MH  - Transcription, Genetic
MH  - Wortmannin
EDAT- 2006/03/21 09:00
MHDA- 2006/09/22 09:00
CRDT- 2006/03/21 09:00
PHST- 2005/01/18 00:00 [received]
PHST- 2005/08/14 00:00 [revised]
PHST- 2005/08/19 00:00 [accepted]
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/09/22 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - S0168-1702(05)00268-6 [pii]
AID - 10.1016/j.virusres.2005.08.018 [doi]
PST - ppublish
SO  - Virus Res. 2006 May;117(2):209-14. doi: 10.1016/j.virusres.2005.08.018. Epub 2006 
      Mar 20.