PMID- 16545913
OWN - NLM
STAT- MEDLINE
DCOM- 20060705
LR  - 20220129
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1760
IP  - 5
DP  - 2006 May
TI  - Identification of new differentiation inducing factors from Dictyostelium 
      discoideum.
PG  - 754-61
AB  - Developing Dictyostelium discoideum amoebae form a stalked fruiting body in which 
      individual cells differentiate into either stalk cells or spores. The major known 
      inducer of stalk cell differentiation is the chlorinated polyketide DIF-1 
      (1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one); however a mutant 
      blocked in the terminal step of DIF-1 biosynthesis still produces one of the 
      prestalk cell subtypes - the pstA cells - as well as some mature stalk cells. We 
      therefore searched for additional stalk cell-inducing factors in the medium 
      supporting development of this mutant. These factors were purified by solvent 
      extraction and HPLC and identified by mass spectroscopy and NMR. The mutant 
      lacked detectable DIF-2 and DIF-3 (the pentanone and deschloro homologues of 
      DIF-1) but four major stalk cell-inducing activities were detected, of which 
      three were identified. Two compounds were predicted intermediates in DIF-1 
      biosynthesis: the desmethyl, and desmethyl-monochloro analogues of DIF-1 
      (dM-DIF-1 and Cl-THPH, respectively), supporting the previously proposed pathway 
      of DIF-1 biosynthesis. The third compound was a novel factor and was identified 
      as 4-methyl-5-pentylbenzene-1,3-diol (MPBD) with the structure confirmed by 
      chemical synthesis. To investigate the potential roles of these compounds as 
      signal molecules, their effects on morphological stalk and spore differentiation 
      were examined in cell culture. All three induced morphological stalk cell 
      differentiation. We found that synthetic MPBD also stimulated spore cell 
      differentiation. Now that these factors are known to be produced and released 
      during development, their biological roles can be pursued further.
FAU - Saito, Tamao
AU  - Saito T
AD  - MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK. 
      tasaito@sci.hokudai.ac.jp
FAU - Taylor, Graham W
AU  - Taylor GW
FAU - Yang, Ji-Chun
AU  - Yang JC
FAU - Neuhaus, David
AU  - Neuhaus D
FAU - Stetsenko, Dmitry
AU  - Stetsenko D
FAU - Kato, Atsushi
AU  - Kato A
FAU - Kay, Robert R
AU  - Kay RR
LA  - eng
GR  - MC_U105115237/MRC_/Medical Research Council/United Kingdom
GR  - MC_U105178934/MRC_/Medical Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060105
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (1-(3,5-dichloro-2,4,6-trihydroxyphenyl)hexane-1-one)
RN  - 0 (1-(3-chloro-2,4,6-trihydroxyphenyl)hexan-1-one)
RN  - 0 (4-methyl-5-pentylbenzene-1,3-diol)
RN  - 0 (Resorcinols)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Dictyostelium/cytology/drug effects/*growth & development
MH  - Mass Spectrometry
MH  - Resorcinols/chemistry/isolation & purification/*pharmacology
EDAT- 2006/03/21 09:00
MHDA- 2006/07/06 09:00
CRDT- 2006/03/21 09:00
PHST- 2005/09/12 00:00 [received]
PHST- 2005/11/28 00:00 [revised]
PHST- 2005/12/06 00:00 [accepted]
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/07/06 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - S0304-4165(05)00392-2 [pii]
AID - 10.1016/j.bbagen.2005.12.006 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2006 May;1760(5):754-61. doi: 10.1016/j.bbagen.2005.12.006. 
      Epub 2006 Jan 5.