PMID- 16546434
OWN - NLM
STAT- MEDLINE
DCOM- 20060905
LR  - 20091119
IS  - 1357-2725 (Print)
IS  - 1357-2725 (Linking)
VI  - 38
IP  - 8
DP  - 2006
TI  - Involvement of protein kinase C and E2F-5 in euxanthone-induced neurite 
      differentiation of neuroblastoma.
PG  - 1393-401
AB  - Euxanthone, a neuritogenic agent isolated from the medicinal herb Polygala 
      caudata, has been shown to induce morphological differentiation and neurite 
      outgrowth in murine neuroblastoma Neuro 2a cells (BU-1 subclone). In order to 
      elucidate the underlying mechanisms of euxanthone-induced neurite outgrowth, a 
      proteomic approach was employed. In the present study, two dimensional (2-D) gel 
      electrophoresis and matrix-assisted laser desorption/ionization-time of flight 
      (MALDI-ToF) mass spectrometry were performed to investigate the alterations in 
      protein expression profile of euxanthone-treated BU-1 cells. Fourteen identified 
      proteins were changed in expression levels after induction of neurite growth. 
      These proteins included participants in transcription and cell cycle regulation, 
      calcium influx and calcium signaling, fatty acid metabolism, cytoskeleton 
      reorganization, casein kinase signal transduction, putative transbilayer 
      amphipath transport and protein biosynthesis. Among the 14 identified proteins, 
      E2F transcription factor 5 (E2F-5) was significantly up-regulated after 
      euxanthone treatment. Go6976, a protein kinase C (PKC) alpha/betaI inhibitor, was 
      found to inhibit neuritogenesis and expression of E2F-5 in the euxanthone-treated 
      BU-1 cells, while SH-6, the Akt/PKB inhibitor, had no inhibitory effect. The gene 
      silencing of E2F-5 by small interfering RNA (siRNA) was found to abolish the 
      euxanthone-induced neurite outgrowth. In conclusion, these results indicated that 
      the transcription factor E2F-5 was actively involved in the regulation of 
      euxanthone-induced neurite outgrowth via PKC pathway.
FAU - Ha, Wai Yan
AU  - Ha WY
AD  - Research and Development Division, School of Chinese Medicine, Hong Kong Baptist 
      University, Kowloon Tong, Hong Kong SAR, China.
FAU - Wu, Pui Kei
AU  - Wu PK
FAU - Kok, Tsz Wai
AU  - Kok TW
FAU - Leung, Kar Wah
AU  - Leung KW
FAU - Mak, Nai Ki
AU  - Mak NK
FAU - Yue, Patrick Ying Kit
AU  - Yue PY
FAU - Ngai, Sai Ming
AU  - Ngai SM
FAU - Tsai, Sau Na
AU  - Tsai SN
FAU - Wong, Ricky Ngok Shun
AU  - Wong RN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060302
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Carbazoles)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (Indoles)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Xanthones)
RN  - 136194-77-9 (Go 6976)
RN  - 529-61-3 (euxanthone)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Carbazoles/pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Line, Tumor
MH  - E2F Transcription Factors/genetics/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gene Silencing
MH  - Indoles/pharmacology
MH  - Neurites/drug effects/*metabolism
MH  - Neuroblastoma/metabolism/pathology
MH  - Protein Kinase C/antagonists & inhibitors/*metabolism
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Proteomics/methods
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Xanthones/*pharmacology
EDAT- 2006/03/21 09:00
MHDA- 2006/09/06 09:00
CRDT- 2006/03/21 09:00
PHST- 2005/10/06 00:00 [received]
PHST- 2006/01/25 00:00 [revised]
PHST- 2006/02/06 00:00 [accepted]
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/09/06 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - S1357-2725(06)00039-2 [pii]
AID - 10.1016/j.biocel.2006.02.002 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2006;38(8):1393-401. doi: 10.1016/j.biocel.2006.02.002. 
      Epub 2006 Mar 2.