PMID- 16546907
OWN - NLM
STAT- MEDLINE
DCOM- 20060601
LR  - 20191210
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 82
IP  - 2
DP  - 2006 Feb
TI  - Evaluation of spectral karyotyping (SKY) in biodosimetry for the triage situation 
      following gamma irradiation.
PG  - 87-96
AB  - PURPOSE: Biological dosimetry in an acute triage situation of radiation exposure 
      is traditionally performed by scoring unstable dicentric chromosomal aberrations 
      after conventional Giemsa staining, and more recently also by detection of 
      chromosomal translocations after chromosome painting by fluorescence in situ 
      hybridization (FISH). By spectral karyotyping (SKY) each chromosome can be 
      painted in an individual colour, permitting the scanning for structural 
      aberrations throughout the genome in each individual metaphase. Here we have 
      evaluated the performance of SKY analysis in a simulated triage situation after 
      gamma irradiation. MATERIALS AND METHODS: Peripheral leukocytes were irradiated 
      by 60Co (0 - 5 Gy) and analysed by SKY, Giemsa staining and FISH painting of 
      chromosomes 1, 2, and 3. RESULTS: At 1 Gy and higher doses, dicentric aberrations 
      (Dic+) as well as classical one- and two-way translocations were found in 
      increasing and dose-dependent frequencies by SKY. The frequency of dicentrics 
      detected by Giemsa was found to be significantly higher than the total 
      aberrations detected by SKY (p<0.001), but did not differ significantly from that 
      of FISH painting. The difference was mainly attributable to the low sensitivity 
      of SKY to detect Dic+ following frequent lack of acentric fragments with matching 
      chromosomal composition. CONCLUSIONS: The findings anticipate that radiation 
      induced chromosomal aberrations may be more complex than expected from 
      conventional and single chromosome painting analyses. While conventional Giemsa 
      staining was found to be the method of choice for the triage situation, it is 
      expected that extended SKY analysis will add to the knowledge of underlying 
      mechanisms for irradiation associated chromosomal aberrations.
FAU - Szeles, Anna
AU  - Szeles A
AD  - Department of Oncology-Pathology, Karolinska University Hospital-Solna, 
      Stockholm, Sweden.
FAU - Joussineau, Sigfried
AU  - Joussineau S
FAU - Lewensohn, Rolf
AU  - Lewensohn R
FAU - Lagercrantz, Svetlana
AU  - Lagercrantz S
FAU - Larsson, Catharina
AU  - Larsson C
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Biological Assay/methods
MH  - Cells, Cultured
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosomes/*radiation effects
MH  - Humans
MH  - Leukocytes/*pathology/*radiation effects
MH  - Male
MH  - Middle Aged
MH  - Radiation Dosage
MH  - Radiometry/*methods
MH  - Reproducibility of Results
MH  - Risk Assessment/methods
MH  - Risk Factors
MH  - Sensitivity and Specificity
MH  - Spectral Karyotyping/*methods
MH  - Triage/*methods
EDAT- 2006/03/21 09:00
MHDA- 2006/06/02 09:00
CRDT- 2006/03/21 09:00
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/06/02 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - K108828408R91530 [pii]
AID - 10.1080/09553000600599767 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2006 Feb;82(2):87-96. doi: 10.1080/09553000600599767.