PMID- 16547701
OWN - NLM
STAT- MEDLINE
DCOM- 20061219
LR  - 20060928
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 72
IP  - 5
DP  - 2006 Oct
TI  - Functional characterization of alcohol oxidases from Aspergillus terreus MTCC 
      6324.
PG  - 906-11
AB  - Short chain alcohol oxidase (SCAO), long chain alcohol oxidase (LCAO), secondary 
      alcohol oxidase (SAO), and aryl alcohol oxidase (AAO) activities were localized 
      in the microsome of Aspergillus terreus during growth of the fungi on 
      n-hexadecane. Zymogram analysis of the microsomes of n-hexadecane-grown cells in 
      polyacrylamide gel electrophoresis showed distinct bands, H4, H3, H2, and H1, in 
      a sequence of their molecular weight (Mr) from high to low. The Mr of the 
      isozymes corresponding to the bands H4, H3, and H2 were close to each other and 
      were higher than 272 kDa. While, the Mr of the isozyme H1 was found to be 
      approximately 48 kDa. H1 gave activity only as SCAO. Although the substrates for 
      other bands were varied, strong (S), medium (M), and weak (W) activity for the 
      bands were as follows: H2: SAO (S), AAO (S), LCAO (M), SCAO (S); H3: LCAO (S), 
      SCAO (S); H4: SCAO (S), LCAO (W), SAO (W). The pH and temperature optima of these 
      isozymes were found to be 8.5+/-0.5 and 30+/-1 degrees C, respectively. The 
      stability of the isozymes was drastically decreased beyond 30 degrees C. The SAO 
      showed 33% enantiomeric excess for the R(-)2-octanol over S(+)2-octanol, which 
      may be correlated with the lower Michaelis-Menten constant (K (M)) values of the 
      enzyme for the R(-)2-octanol than the S(+)2-octanol. The fluorescence emission 
      spectra of the chromatographically purified SCAO at 443 nm excitation were 
      similar to that obtained with authentic flavin adenine dinucleotide.
FAU - Kumar, A Kiran
AU  - Kumar AK
AD  - Department of Biotechnology, Indian Institute of Technology Guwahati, North 
      Guwahati, Assam 781039, India.
FAU - Goswami, Pranab
AU  - Goswami P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060318
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - EC 1.1.- (Alcohol Oxidoreductases)
SB  - IM
MH  - Alcohol Oxidoreductases/chemistry/*metabolism
MH  - Aspergillus/*enzymology
MH  - Enzyme Stability
MH  - Substrate Specificity
MH  - Temperature
EDAT- 2006/03/21 09:00
MHDA- 2006/12/21 09:00
CRDT- 2006/03/21 09:00
PHST- 2006/01/10 00:00 [received]
PHST- 2006/02/15 00:00 [accepted]
PHST- 2006/02/15 00:00 [revised]
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2006/12/21 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - 10.1007/s00253-006-0381-y [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2006 Oct;72(5):906-11. doi: 10.1007/s00253-006-0381-y. 
      Epub 2006 Mar 18.