PMID- 16547744
OWN - NLM
STAT- MEDLINE
DCOM- 20070620
LR  - 20161128
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 385
IP  - 1
DP  - 2006 May
TI  - Detection of residual bacitracin A, colistin A, and colistin B in milk and animal 
      tissues by liquid chromatography tandem mass spectrometry.
PG  - 181-8
AB  - Liquid chromatography coupled with electrospray ionization tandem mass 
      spectrometry (LC-MS/MS) was applied to the determination of residual bacitracin 
      A, colistin A, and colistin B in milk and animal tissue samples. Prior to 
      instrumental analysis, samples were subjected to acid extraction followed by 
      solid-phase cleanup using Strata-X cartridges. Mass spectral acquisitions were 
      performed under selective multiple reaction monitoring (MRM) mode at m/z 199 and 
      670 from triply charged precursors of bacitracin A (m/z 475); m/z 385 and 379 
      from triply charged precursors of colistin A (m/z 391); and m/z 380 and 374 from 
      triply charged precursors of colistin B (m/z 386). Method precision was evaluated 
      from spike recovery of samples fortified at concentrations corresponding to 2/5 
      of the maximum residue limits (MRLs) for each of the analytes under study. 
      Intra-day and inter-day variations were found to range from 90.9 to 104% with 
      relative standard deviation (RSD) <6.5%, and from 90.1 to 106% with RSD <9.1%, 
      respectively. Limits of quantification (LOQs) were defined as the spiking 
      concentrations at 2/5 MRL, and limits of detection (LODs) were 10-47 microg 
      kg(-1) for bacitracin A, 1-16 microg kg(-1) for colistin A, and 6-14 microg 
      kg(-1) for colistin B in milk and animal tissues. The presented method has good 
      precision and high sensitivity and was applied as a fast screening protocol and a 
      quantitative tool for monitoring of the concerned polypeptides in foods as part 
      of a surveillance program.
FAU - Wan, Eric Chun-hong
AU  - Wan EC
AD  - Analytical and Advisory Services, Government Laboratory of the Hong Kong Special 
      Administrative Region (HKSAR), Homantin Government Offices, Hong Kong, China.
FAU - Ho, Clare
AU  - Ho C
FAU - Sin, Della Wai-mei
AU  - Sin DW
FAU - Wong, Yiu-chung
AU  - Wong YC
LA  - eng
PT  - Journal Article
DEP - 20060318
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 1405-87-4 (Bacitracin)
RN  - DDA3RRX0P7 (bacitracin A)
RN  - Z67X93HJG1 (Colistin)
SB  - IM
MH  - Animals
MH  - Bacitracin/*analysis
MH  - Cattle
MH  - Chromatography, Liquid/*methods
MH  - Colistin/*analysis
MH  - Drug Residues/*analysis
MH  - Liver/chemistry
MH  - Milk/*chemistry
MH  - Molecular Structure
MH  - Muscle, Skeletal/chemistry
MH  - Poultry
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2006/03/21 09:00
MHDA- 2007/06/21 09:00
CRDT- 2006/03/21 09:00
PHST- 2005/11/13 00:00 [received]
PHST- 2006/01/19 00:00 [accepted]
PHST- 2005/12/31 00:00 [revised]
PHST- 2006/03/21 09:00 [pubmed]
PHST- 2007/06/21 09:00 [medline]
PHST- 2006/03/21 09:00 [entrez]
AID - 10.1007/s00216-006-0325-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2006 May;385(1):181-8. doi: 10.1007/s00216-006-0325-5. Epub 
      2006 Mar 18.