PMID- 16548684
OWN - NLM
STAT- MEDLINE
DCOM- 20060503
LR  - 20181201
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 12
IP  - 2
DP  - 2006 Feb
TI  - Osteogenic induction of human periodontal ligament fibroblasts under two- and 
      three-dimensional culture conditions.
PG  - 257-66
AB  - Human periodontal ligament fibroblasts (hPDLF) play a key role in the 
      regeneration of periodontal compartment during guided tissue regeneration 
      procedures. This property is attributed to the progenitor cell subsets residing 
      in the area. The aim of this study was to investigate whether hPDLFs could 
      undergo an osteogenic differentiation under two- and three-dimensional (2D and 
      3D) culture conditions upon osteogenic induction. hPDLFs were isolated from six 
      healthy donors, cultured, and expanded according to standard protocols. Then, 
      three osteogenic culture conditions (dexamethasone, ascorbic acid, and 
      beta-glycerophosphate) were established: 1) 2D culture as single-cell monolayer, 
      2) 3D-static culture on mineralized poly(DL-lactic-co-glycolic acid) (PLGA) 
      scaffold, and 3) 3D culture on mineralized PLGA scaffold inside the NASA-approved 
      bioreactor stimulating microgravity conditions. After 21 days of osteogenic 
      induction, the majority of monolayer cultures had undergone differentiation 
      toward osteogenic lineage, as indicated by morphological changes, mineralization 
      assay, and some phenotypical properties. However, immunohistochemistry revealed 
      that the scaffold cultures expressed higher levels of osteogenic marker proteins 
      compared with that of the monolayers. Secondly, hPDLF-PLGA constructs in 
      bioreactor showed an increased expression of osteopontin and osteocalcin compared 
      with that of static 3D culture after 21 days. Results indicate that human 
      periodontal ligament contains a subpopulation of cells capable of undergoing 
      osteogenic differentiation and presumably contributing to regeneration of bone 
      defects in the adjacent area. Human PDLF-seeded mineralized PLGA scaffold in 
      microgravity bioreactor may be used to support osteogenic differentiation in 
      vitro. Thus, this system may offer new potential benefits as a tool for 
      periodontal tissue engineering.
FAU - Inanc, Bulend
AU  - Inanc B
AD  - Tissue Engineering and Biomaterials Laboratory, Biotechnology Institute and 
      Faculty of Science, Ankara University, Ankara, Turkey.
FAU - Elcin, A Eser
AU  - Elcin AE
FAU - Elcin, Y Murat
AU  - Elcin YM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Biomarkers)
RN  - 0 (Polymers)
RN  - 0 (SPP1 protein, human)
RN  - 0 (Sialoglycoproteins)
RN  - 104982-03-8 (Osteocalcin)
RN  - 106441-73-0 (Osteopontin)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Ascorbic Acid/pharmacology
MH  - Biomarkers/metabolism
MH  - Bioreactors
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Fibroblasts/*drug effects/ultrastructure
MH  - Humans
MH  - Immunohistochemistry
MH  - Lactic Acid/chemistry
MH  - Osteocalcin/metabolism
MH  - Osteogenesis/*drug effects
MH  - Osteopontin
MH  - Periodontal Ligament/cytology/*drug effects
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/chemistry
MH  - Sialoglycoproteins/metabolism
MH  - Time Factors
MH  - Tissue Engineering/methods
EDAT- 2006/03/22 09:00
MHDA- 2006/05/04 09:00
CRDT- 2006/03/22 09:00
PHST- 2006/03/22 09:00 [pubmed]
PHST- 2006/05/04 09:00 [medline]
PHST- 2006/03/22 09:00 [entrez]
AID - 10.1089/ten.2006.12.257 [doi]
PST - ppublish
SO  - Tissue Eng. 2006 Feb;12(2):257-66. doi: 10.1089/ten.2006.12.257.