PMID- 16548914
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20211203
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 77
IP  - 1
DP  - 2006 Jul
TI  - Deregulation of cyclin D2 by juxtaposition with T-cell receptor alpha/delta locus 
      in t(12;14)(p13;q11)-positive childhood T-cell acute lymphoblastic leukemia.
PG  - 27-34
AB  - OBJECTIVES: The t(12;14)(p13;q11)--a recurrent translocation in childhood T-cell 
      acute lymphoblastic leukemia (T-ALL)--has very recently been molecularly 
      characterized in one case, which displayed overexpression of the cyclin D2 gene 
      (CCND2). PATIENTS AND METHODS: We have characterized two pediatric 
      t(12;14)-positive T-ALLs using fluorescence in situ hybridization (FISH), cDNA 
      microarray, and real-time polymerase chain reaction (PCR). RESULTS: FISH revealed 
      breakpoints (BPs) in the T-cell receptor alpha/delta locus (14q11) and in the 
      vicinity of the CCND2 gene at 12p13. To investigate the expression of genes in 
      12p13, cDNA microarray analysis was performed. Expression data for eight genes, 
      including CCND2, surrounding the 12p BP were compared with those in other T-ALLs. 
      The t(12;14)-positive T-ALL displayed an increased expression of CCND2 compared 
      to the controls, whereas the expression of the other genes was similar in all 
      T-ALLs. Expression of CCND2 and two additional genes (PARP11 and FGF23), close to 
      the 12p BP, was investigated with real-time PCR of the two t(12;14)-positive 
      cases and four controls. Neither PARP11 nor FGF23 displayed expression 
      differences among the T-ALLs, whereas CCND2 was clearly overexpressed in both 
      t(12;14)-positive cases as compared to the mean expression level in the controls. 
      CONCLUSION: We have confirmed, in two additional cases, that the recurrent 
      T-ALL-associated t(12;14) results in overexpression of cyclin D2. The t(12;14) is 
      the first neoplasia-associated translocation shown to result in overexpression of 
      cyclin D2. Furthermore, it is the first example of a T-cell neoplasm with a 
      targeted deregulation of a member of a cyclin-encoding gene family.
FAU - Karrman, Kristina
AU  - Karrman K
AD  - Department of Clinical Genetics, University Hospital, Lund, Sweden. 
      Kristina.Karrman@med.lu.se
FAU - Andersson, Anna
AU  - Andersson A
FAU - Bjorgvinsdottir, Helga
AU  - Bjorgvinsdottir H
FAU - Strombeck, Bodil
AU  - Strombeck B
FAU - Lassen, Carin
AU  - Lassen C
FAU - Olofsson, Tor
AU  - Olofsson T
FAU - Nguyen-Khac, Florence
AU  - Nguyen-Khac F
FAU - Berger, Roland
AU  - Berger R
FAU - Bernard, Olivier
AU  - Bernard O
FAU - Fioretos, Thoas
AU  - Fioretos T
FAU - Johansson, Bertil
AU  - Johansson B
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060317
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (CCND2 protein, human)
RN  - 0 (Cyclin D2)
RN  - 0 (Cyclins)
RN  - 0 (FGF23 protein, human)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
RN  - 7Q7P4S7RRE (Fibroblast Growth Factor-23)
SB  - IM
MH  - Adolescent
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 14
MH  - Cyclin D2
MH  - Cyclins/*genetics/metabolism
MH  - Female
MH  - Fibroblast Growth Factor-23
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics/metabolism
MH  - Receptors, Antigen, T-Cell, gamma-delta/*genetics/metabolism
MH  - *Translocation, Genetic
EDAT- 2006/03/22 09:00
MHDA- 2006/08/25 09:00
CRDT- 2006/03/22 09:00
PHST- 2006/03/22 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2006/03/22 09:00 [entrez]
AID - EJH651 [pii]
AID - 10.1111/j.0902-4441.2006.t01-1-EJH2553.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2006 Jul;77(1):27-34. doi: 
      10.1111/j.0902-4441.2006.t01-1-EJH2553.x. Epub 2006 Mar 17.