PMID- 16549062
OWN - NLM
STAT- MEDLINE
DCOM- 20060915
LR  - 20191003
IS  - 0014-4835 (Print)
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 83
IP  - 2
DP  - 2006 Aug
TI  - Expression and localization of sterol 27-hydroxylase (CYP27A1) in monkey retina.
PG  - 465-9
AB  - Sterol 27-hydroxylase (CYP27A1) is a mitochondrial P-450 enzyme with broad 
      substrate specificity for C27 sterols including 7-ketocholesterol (7kCh). CYP27A1 
      is widely expressed in human tissues but has not been previously demonstrated in 
      the retina. In this study, we examined the expression and localization of CYP27A1 
      in the monkey retina where it localized mainly to the photoreceptor inner 
      segments. CYP27A1 was also observed in Muller cells with faint immuno staining 
      detected in the RPE and choriocapillaris. We also determined that the 
      27-hydroxylation of 7-ketocholesterol (27OH7kCh) rendered it non-toxic to 
      cultured RPE cells. Moreover, the 27OH7kCh when mixed with 7-ketocholesterol 
      significantly reduced the toxicity of 7-ketocholesterol. These data, when taken 
      in context of the known functions of CYP27A1 imply that expression in the retina 
      serves to modify the biological activity of oxidized sterols that are either 
      transported or generated locally by photo-oxidation.
FAU - Lee, Jung Wha
AU  - Lee JW
AD  - Section on Mechanisms of Retinal Diseases, Laboratory of Retinal Cell and 
      Molecular Biology, National Eye Institute, National Institutes of Health, 
      Bethesda, MD 20892, USA.
FAU - Fuda, Hirotoshi
AU  - Fuda H
FAU - Javitt, Norman B
AU  - Javitt NB
FAU - Strott, Charles A
AU  - Strott CA
FAU - Rodriguez, Ignacio R
AU  - Rodriguez IR
LA  - eng
GR  - ZIA EY000307-15/Intramural NIH HHS/United States
PT  - Letter
PT  - Research Support, N.I.H., Intramural
DEP - 20060320
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Ketocholesterols)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.15.15 (CYP27A1 protein, human)
RN  - EC 1.14.15.15 (Cholestanetriol 26-Monooxygenase)
RN  - O7676FE78M (7-ketocholesterol)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cholestanetriol 26-Monooxygenase
MH  - Enzyme Inhibitors/metabolism
MH  - Hydroxylation
MH  - Immunohistochemistry/methods
MH  - Ketocholesterols/metabolism
MH  - Macaca mulatta
MH  - Photoreceptor Cells, Vertebrate/chemistry
MH  - Pigment Epithelium of Eye/chemistry
MH  - Retina/*chemistry
MH  - Steroid Hydroxylases/*analysis
PMC - PMC2806429
MID - NIHMS166790
EDAT- 2006/03/22 09:00
MHDA- 2006/09/16 09:00
PMCR- 2010/01/13
CRDT- 2006/03/22 09:00
PHST- 2005/11/08 00:00 [received]
PHST- 2005/11/11 00:00 [accepted]
PHST- 2006/03/22 09:00 [pubmed]
PHST- 2006/09/16 09:00 [medline]
PHST- 2006/03/22 09:00 [entrez]
PHST- 2010/01/13 00:00 [pmc-release]
AID - S0014-4835(06)00078-9 [pii]
AID - 10.1016/j.exer.2005.11.018 [doi]
PST - ppublish
SO  - Exp Eye Res. 2006 Aug;83(2):465-9. doi: 10.1016/j.exer.2005.11.018. Epub 2006 Mar 
      20.