PMID- 16549269
OWN - NLM
STAT- MEDLINE
DCOM- 20060803
LR  - 20131121
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 139
IP  - 3
DP  - 2006
TI  - Characterization of the putative cholesterol transport protein metastatic lymph 
      node 64 in the brain.
PG  - 1031-8
AB  - Intracellular management of cholesterol is a critical process in the brain. 
      Deficits with cholesterol transport and storage are linked to neurodegenerative 
      disorders such as Neimann-Pick disease type C and Alzheimer's disease. One 
      protein putatively involved in cholesterol transport is metastatic lymph node 64 
      (MLN64). MLN64 localizes to late endosomes which are part of the cholesterol 
      internalization pathway. However, a detailed pattern of MLN64 expression in the 
      brain is unclear. Using immunocytochemical and immunoblot analyses, we 
      demonstrated the presence of MLN64 in several tissue types and various regions 
      within the brain. MLN64 immunostaining in the CNS was heterogeneous, indicating 
      selective expression in discrete specific cell populations and regions. MLN64 
      immunoreactivity was detected in glia and neurons, which displayed intracellular 
      labeling consistent with an endosomal localization. Although previous studies 
      suggested that MLN64 may promote steroid production in the brain, MLN64 
      immunoreactivity did not colocalize with steroidogenic cells in the CNS. These 
      results demonstrate that MLN64 is produced in the mouse and human CNS in a 
      restricted pattern of expression, suggesting that MLN64 serves a cell-specific 
      function in cholesterol transport.
FAU - King, S R
AU  - King SR
AD  - Scott Department of Urology, Room N730, Baylor College of Medicine, One Baylor 
      Plaza, Houston, TX 77030, USA. srking@bcm.tmc.edu
FAU - Smith, A G A
AU  - Smith AG
FAU - Alpy, F
AU  - Alpy F
FAU - Tomasetto, C
AU  - Tomasetto C
FAU - Ginsberg, S D
AU  - Ginsberg SD
FAU - Lamb, D J
AU  - Lamb DJ
LA  - eng
GR  - AG17617/AG/NIA NIH HHS/United States
GR  - DK61548/DK/NIDDK NIH HHS/United States
GR  - NS43939/NS/NINDS NIH HHS/United States
GR  - NS48447/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060323
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (STARD3 protein, human)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain/*metabolism
MH  - Carrier Proteins/*biosynthesis
MH  - Cholesterol/*metabolism
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Membrane Proteins/*biosynthesis
MH  - Mice
MH  - Neuroglia/metabolism
MH  - Neurons/metabolism
MH  - Protein Transport/physiology
EDAT- 2006/03/22 09:00
MHDA- 2006/08/04 09:00
CRDT- 2006/03/22 09:00
PHST- 2005/03/11 00:00 [received]
PHST- 2005/12/20 00:00 [revised]
PHST- 2006/01/15 00:00 [accepted]
PHST- 2006/03/22 09:00 [pubmed]
PHST- 2006/08/04 09:00 [medline]
PHST- 2006/03/22 09:00 [entrez]
AID - S0306-4522(06)00098-4 [pii]
AID - 10.1016/j.neuroscience.2006.01.063 [doi]
PST - ppublish
SO  - Neuroscience. 2006;139(3):1031-8. doi: 10.1016/j.neuroscience.2006.01.063. Epub 
      2006 Mar 23.