PMID- 16552729
OWN - NLM
STAT- MEDLINE
DCOM- 20061207
LR  - 20131121
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 98
IP  - 6
DP  - 2006 Aug 15
TI  - Comparative proteomic analysis of apoptosis induced by sodium selenite in human 
      acute promyelocytic leukemia NB4 cells.
PG  - 1495-506
AB  - Selenium (Se) is an essential trace element possessing anticarcinogenic 
      properties. Sodium selenite (Na2SeO3) induced apoptosis in human acute 
      promyelocytic leukemia (APL) cell line NB4 with dose and time dependency. In this 
      study, proteomic techniques were used to study the apoptosis of NB4 cells induced 
      by sodium selenite. Twenty-six downregulated and four upregulated proteins were 
      identified, which exhibited a 1.5-fold change or greater. The identified proteins 
      included key regulators of signal transduction such as Rho GDP dissociation 
      inhibitor (Rho GDI) alpha and beta members of the MAPK family, and proteins 
      involved in the regulation of c-fos or c-myc expression. Importantly, the 
      identified proteins, hnRNP D0B and Rho GDI beta, which were related with the 
      regulation of c-myc, c-fos, and c-jun, were determined by reverse 
      transcription-polymerase chain reaction (RT-PCR) to confirm their downregulation 
      in proteomic study. Western blot analysis and RT-PCR were then performed on three 
      associated proteins: c-Myc, c-Fos, and c-Jun, and their expression were observed 
      to be significantly downregulated. Results showed that certain regulation 
      involved in c-myc, c-fos, and c-jun was present in the apoptosis, and the c-Myc 
      dependent-on and Jun N-terminal kinase (JNK) pathway also play roles.
CI  - (c) 2006 Wiley-Liss, Inc.
FAU - Dong, Hua
AU  - Dong H
AD  - National Laboratory of Medical Molecular Biology, Institute of Basic Medical 
      Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Ying, Tianyi
AU  - Ying T
FAU - Li, Ting
AU  - Li T
FAU - Cao, Tingming
AU  - Cao T
FAU - Wang, Junjun
AU  - Wang J
FAU - Yuan, Jing
AU  - Yuan J
FAU - Feng, Erling
AU  - Feng E
FAU - Han, Bingshe
AU  - Han B
FAU - Hua, Fangyuan
AU  - Hua F
FAU - Yang, Yang
AU  - Yang Y
FAU - Yuan, Jiangang
AU  - Yuan J
FAU - Wang, Hengliang
AU  - Wang H
FAU - Xu, Caimin
AU  - Xu C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Proteome)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - HIW548RQ3W (Sodium Selenite)
SB  - IM
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Leukemia, Promyelocytic, Acute/*metabolism
MH  - Mass Spectrometry
MH  - Proteome/*metabolism
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Sodium Selenite/*pharmacology
EDAT- 2006/03/23 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/03/23 09:00
PHST- 2006/03/23 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/03/23 09:00 [entrez]
AID - 10.1002/jcb.20755 [doi]
PST - ppublish
SO  - J Cell Biochem. 2006 Aug 15;98(6):1495-506. doi: 10.1002/jcb.20755.