PMID- 16553217
OWN - NLM
STAT- MEDLINE
DCOM- 20090331
LR  - 20120611
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 33
IP  - 3
DP  - 2006 Mar
TI  - Localization of S genes on extended DNA fibers (EDFs) in Brassica oleracea by 
      high-resolution FISH.
PG  - 277-84
AB  - The compactness of plant chromosome and the structures of plant cell wall and 
      cytoplasm pose a great resistance to fluorescence in situ hybridization (FISH), 
      and consequently many new methods for improving spatial resolution are being 
      exploited to overcome these problems. However, for plants with small chromosomes 
      like rice and Brassica, there are still many difficulties. In this article a new 
      and effective technique for preparation of extended DNA fibers (EDFs), using a 
      series of treatments to prophase I chromosomes of Brassica oleracea PMCs, is 
      presented. This technique allows longitudinal extension of the chromosomes 30-107 
      times longer than those of their metaphase counterparts. The length of the 
      extended DNA fibers is between 89 microm and 273 microm, and the space resolution 
      is 42.8-53.0 kb. Stretching ratios were assessed in a number of FISH experiments 
      with super-stretched chromosomes from meiotic prophase I nuclei of B. olerecea. 
      Through FISH to EDFs of pachytene chromosomes hybridized in situ with SRK 
      (S-locus receptor kinase) and SPII (S-locus protein II) probes, for the first 
      time we localized the accurate positions of S-locus and quantitatively analyzed 
      the features of S genes in B. oleracea genome to show all S genes were 
      single-copied. In addition, the length between two linked genes was measured to 
      be about one micron. As a result, the highest space resolution which was about 4 
      kb was obtained.
FAU - Yang, Kun
AU  - Yang K
AD  - College of Agronomy and Life Sciences, Southwest University, Chongqing 400716, 
      China. ykun0827@163.com
FAU - Qi, Hong-Yan
AU  - Qi HY
FAU - Zhu, Li-Quan
AU  - Zhu LQ
FAU - Wang, Xiao-Jia
AU  - Wang XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (DNA, Plant)
RN  - 0 (Glycoproteins)
RN  - 0 (Plant Proteins)
RN  - 0 (self-incompatibility locus specific glycoprotein, Brassica)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (S-receptor kinase)
SB  - IM
MH  - Brassica/*genetics
MH  - Chromosomes, Plant/genetics
MH  - DNA, Plant/*genetics
MH  - Glycoproteins/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Physical Chromosome Mapping
MH  - Plant Proteins/*genetics
MH  - Protein Kinases/*genetics
EDAT- 2006/03/24 09:00
MHDA- 2009/04/01 09:00
CRDT- 2006/03/24 09:00
PHST- 2006/03/24 09:00 [pubmed]
PHST- 2009/04/01 09:00 [medline]
PHST- 2006/03/24 09:00 [entrez]
AID - S0379-4172(06)60051-6 [pii]
AID - 10.1016/S0379-4172(06)60051-6 [doi]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2006 Mar;33(3):277-84. doi: 10.1016/S0379-4172(06)60051-6.