PMID- 16553735
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20061115
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 100
IP  - 4
DP  - 2006 Apr
TI  - Immunocapture UPPCR combined with DGGE for rapid detection of Shigella species.
PG  - 795-9
AB  - AIMS: To develop an immunocapture universal primer PCR (iUPPCR) combined with 
      denaturing gradient gel electrophoresis (DGGE) and evaluate it as a method 
      permitting rapid detection of Shigella species and their serotypes. METHODS AND 
      RESULTS: This method amplifying the conserved regions of bacterial 16S rRNA genes 
      of different species or serotypes of Shigella dysentery bacilli captured and 
      enriched by polyvalent antibodies can detect and distinguish causative pathogens 
      rapidly. Four serotypes from three Shigella species including Shigella 
      dysenteriae serotype 1, Shigella boydii serotype 1, Shigella flexneri serotypes 
      1a and 3a were examined. CONCLUSION: Our approach could be adopted for not only 
      axenic bacterial population but also mixed communities and achieve rapid 
      detection of various bacteria from the same genus or species in one sample. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: The iUPPCR-DGGE method was shown to be more 
      convenient than serotype-specific-antibody-based method of iUPPCR for Shigella 
      species detection and it could be also applied to the quick detection for other 
      kinds of pathogens with many serotypes.
FAU - Ji, N N
AU  - Ji NN
AD  - Department of Biology, School of Life Sciences, Xiamen University, Xiamen, 
      Fujian, PR China.
FAU - Liu, M M
AU  - Liu MM
FAU - Huang, X R
AU  - Huang XR
FAU - Zhen, J
AU  - Zhen J
FAU - Li, S S
AU  - Li SS
FAU - Jiang, S
AU  - Jiang S
FAU - Yu, H H
AU  - Yu HH
FAU - Wang, S Y
AU  - Wang SY
FAU - Peng, X X
AU  - Peng XX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - Genes, Bacterial/genetics
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Serotyping/methods
MH  - Shigella/classification/*isolation & purification
MH  - Shigella boydii/classification/isolation & purification
MH  - Shigella dysenteriae/classification/isolation & purification
MH  - Shigella flexneri/classification/isolation & purification
EDAT- 2006/03/24 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/03/24 09:00
PHST- 2006/03/24 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/03/24 09:00 [entrez]
AID - JAM2836 [pii]
AID - 10.1111/j.1365-2672.2006.02836.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2006 Apr;100(4):795-9. doi: 10.1111/j.1365-2672.2006.02836.x.