PMID- 16553822
OWN - NLM
STAT- MEDLINE
DCOM- 20060620
LR  - 20081121
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 46
IP  - 3
DP  - 2006 Apr
TI  - An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium 
      longum inhibits IgE production in vitro.
PG  - 461-9
AB  - The immunostimulatory oligodeoxynucleotide (ODN) BL07 
      (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic 
      strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte 
      proliferation and induced interleukin-12 (IL-12) production in macrophage-like 
      J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) 
      significantly inhibited immunoglobulin E (IgE) production and stimulated 
      interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 
      secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs 
      also significantly inhibited production of IgE in purified murine B cells in the 
      presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 
      and BL07S in preventing IgE-related immune responses and the possible involvement 
      of ODN BL07 in the antiallergic efficacy of B. longum BB536.
FAU - Takahashi, Noritoshi
AU  - Takahashi N
AD  - Food Research and Development Laboratory, Morinaga Milk Industry Co., Ltd, Zama, 
      Kanagawa, Japan. no_takah@morinagamilk.co.jp
FAU - Kitazawa, Haruki
AU  - Kitazawa H
FAU - Shimosato, Takeshi
AU  - Shimosato T
FAU - Iwabuchi, Noriyuki
AU  - Iwabuchi N
FAU - Xiao, Jin-Zhong
AU  - Xiao JZ
FAU - Iwatsuki, Keiji
AU  - Iwatsuki K
FAU - Kokubo, Sadayuki
AU  - Kokubo S
FAU - Saito, Tadao
AU  - Saito T
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (DNA, Bacterial)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (NF-kappa B)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/cytology/immunology
MH  - Base Sequence
MH  - Bifidobacterium/*genetics/*immunology
MH  - Cell Proliferation
MH  - DNA, Bacterial/chemistry/genetics/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoglobulin E/*biosynthesis/immunology
MH  - Immunoglobulin Isotypes/immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-12/immunology
MH  - Interleukin-4/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - NF-kappa B/immunology
MH  - Oligodeoxyribonucleotides/*genetics/immunology/isolation & purification
MH  - Probiotics/*pharmacology
MH  - Sequence Analysis, DNA
MH  - Specific Pathogen-Free Organisms
EDAT- 2006/03/24 09:00
MHDA- 2006/06/21 09:00
CRDT- 2006/03/24 09:00
PHST- 2006/03/24 09:00 [pubmed]
PHST- 2006/06/21 09:00 [medline]
PHST- 2006/03/24 09:00 [entrez]
AID - FIM064 [pii]
AID - 10.1111/j.1574-695X.2006.00064.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2006 Apr;46(3):461-9. doi: 
      10.1111/j.1574-695X.2006.00064.x.