PMID- 16553830
OWN - NLM
STAT- MEDLINE
DCOM- 20060628
LR  - 20131121
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 257
IP  - 1
DP  - 2006 Apr
TI  - Mycobacterium marinum biofilm formation reveals cording morphology.
PG  - 43-9
AB  - Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically 
      relevant pathogens has warranted the study of these ubiquitous organisms in the 
      context of their likely environmental niche, the biofilm. We assayed the NTM 
      bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for 
      biofilm formation on different surfaces over time using three different methods. 
      Using the MBEC system, biofilm development occurred continually over the 14-day 
      culture period reaching a mature or stable biofilm state after 7 days 
      postinoculation. Quantification of M. marinum biofilm formation on high-density 
      polyethylene (HDPE), polycarbonate (PC) and silicon (Si) coupons over a 14-day 
      period was evaluated using a continuous flow reactor system. M. marinum developed 
      biofilms on all of the surfaces tested. However, substantially more biofilm 
      accumulated on the silicon than on the other substrates (Si>HDPE>PC) under the 
      same growth conditions indicating that silicon was the most effective substratum 
      studied for the generation of M. marinum biofilms and suggesting a correlation 
      between surface hydrophobicity and attachment. Finally, confocal laser scanning 
      microscopy was used to visualize M. marinum biofilm development in situ over time 
      and revealed an unusual biofilm ultrastructure. Large cell clusters attached to 
      the surface grew in parallel sinuous arrays of cells that formed large cords.
FAU - Hall-Stoodley, Luanne
AU  - Hall-Stoodley L
AD  - Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA, 
      USA.
FAU - Brun, Olivier S
AU  - Brun OS
FAU - Polshyna, Ganna
AU  - Polshyna G
FAU - Barker, Lucia P
AU  - Barker LP
LA  - eng
GR  - AI060862/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Culture Media)
RN  - 0 (Polycarboxylate Cement)
RN  - 25766-59-0 (polycarbonate)
RN  - 9002-88-4 (Polyethylene)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Animals
MH  - Bacterial Adhesion
MH  - Biofilms/*growth & development
MH  - Bioreactors
MH  - Colony Count, Microbial
MH  - Culture Media
MH  - Microscopy, Confocal
MH  - Mycobacterium marinum/cytology/*growth & development
MH  - Polycarboxylate Cement
MH  - Polyethylene
MH  - Silicon
EDAT- 2006/03/24 09:00
MHDA- 2006/06/29 09:00
CRDT- 2006/03/24 09:00
PHST- 2006/03/24 09:00 [pubmed]
PHST- 2006/06/29 09:00 [medline]
PHST- 2006/03/24 09:00 [entrez]
AID - FML143 [pii]
AID - 10.1111/j.1574-6968.2006.00143.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2006 Apr;257(1):43-9. doi: 10.1111/j.1574-6968.2006.00143.x.