PMID- 16554469
OWN - NLM
STAT- MEDLINE
DCOM- 20060414
LR  - 20191008
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 26
IP  - 12
DP  - 2006 Mar 22
TI  - Chemokines regulate the migration of neural progenitors to sites of 
      neuroinflammation.
PG  - 3182-91
AB  - Many studies have shown that transplanted or endogenous neural progenitor cells 
      will migrate toward damaged areas of the brain. However, the mechanism underlying 
      this effect is not clear. Here we report that, using hippocampal slice cultures, 
      grafted neural progenitor cells (NPs) migrate toward areas of neuroinflammation 
      and that chemokines are a major regulator of this process. Migration of NPs was 
      observed after injecting an inflammatory stimulus into the area of the fimbria 
      and transplanting enhanced green fluorescent protein (EGFP)-labeled NPs into the 
      dentate gyrus of cultured hippocampal slices. Three to 7 d after transplantation, 
      EGFP-NPs in control slices showed little tendency to migrate and had 
      differentiated into neurons and glia. In contrast, in slices injected with 
      inflammatory stimuli, EGFP-NPs migrated toward the site of the injection. NPs in 
      these slices also survived less well. The inflammatory stimuli used were a 
      combination of the cytokines tumor necrosis factor-alpha and interferon-gamma, 
      the bacterial toxin lipopolysaccharide, the human immunodeficiency virus-1 coat 
      protein glycoprotein 120, or a beta-amyloid-expressing adenovirus. We showed that 
      these inflammatory stimuli increased the synthesis of numerous chemokines and 
      cytokines by hippocampal slices. When EGFP-NPs from CC chemokine receptor CCR2 
      knock-out mice were transplanted into slices, they exhibited little migration 
      toward sites of inflammation. Similarly, wild-type EGFP-NPs exhibited little 
      migration toward inflammatory sites when transplanted into slices prepared from 
      monocyte chemoattractant protein-1 (MCP-1) knock-out mice. These data indicate 
      that factors secreted by sites of neuroinflammation are attractive to neural 
      progenitors and suggest that chemokines such as MCP-1 play an important role in 
      this process.
FAU - Belmadani, Abdelhak
AU  - Belmadani A
AD  - Department of Molecular Pharmacology and Biological Chemistry, Northwestern 
      University Medical School, Chicago, Illinois 60611, USA.
FAU - Tran, Phuong B
AU  - Tran PB
FAU - Ren, Dongjun
AU  - Ren D
FAU - Miller, Richard J
AU  - Miller RJ
LA  - eng
GR  - R01 DA013141/DA/NIDA NIH HHS/United States
GR  - R01 DA013141-04/DA/NIDA NIH HHS/United States
GR  - R37MH040165/MH/NIMH NIH HHS/United States
GR  - R01DA013141/DA/NIDA NIH HHS/United States
GR  - R01NS043095/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (CCR2 protein, human)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain Tissue Transplantation/methods
MH  - Cell Differentiation/physiology
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics
MH  - Chemokines/immunology/*metabolism
MH  - Cytokines/immunology/metabolism
MH  - Encephalitis/immunology/*metabolism/physiopathology
MH  - Graft Survival/physiology
MH  - Green Fluorescent Proteins
MH  - Hippocampus/cytology/immunology/metabolism
MH  - Inflammation Mediators/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred ICR
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Neurons/immunology/*metabolism
MH  - Organ Culture Techniques
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/genetics
MH  - Stem Cell Transplantation/methods
MH  - Stem Cells/immunology/*metabolism
PMC - PMC2740990
MID - NIHMS103228
EDAT- 2006/03/24 09:00
MHDA- 2006/04/15 09:00
PMCR- 2006/09/22
CRDT- 2006/03/24 09:00
PHST- 2006/03/24 09:00 [pubmed]
PHST- 2006/04/15 09:00 [medline]
PHST- 2006/03/24 09:00 [entrez]
PHST- 2006/09/22 00:00 [pmc-release]
AID - 26/12/3182 [pii]
AID - 10.1523/JNEUROSCI.0156-06.2006 [doi]
PST - ppublish
SO  - J Neurosci. 2006 Mar 22;26(12):3182-91. doi: 10.1523/JNEUROSCI.0156-06.2006.