PMID- 16554736
OWN - NLM
STAT- MEDLINE
DCOM- 20060710
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 19
IP  - 4
DP  - 2006 Apr
TI  - Epithelial growth factor receptor status in primary and recurrent ovarian cancer.
PG  - 607-10
AB  - Success of epidermal growth factor receptor (EGFR) targeting agents in different 
      cancer types is related to EGFR gene mutations and/or copy number gains. We 
      investigated the EGFR gene status and protein expression by DNA mutational 
      analysis, fluorescence in situ hybridization (FISH), and immunohistochemistry in 
      tumor tissues from 80 patients with primary and corresponding recurrent ovarian 
      serous carcinomas. The patients were classified into six groups with ascending 
      EGFR gene copy numbers. EGFR amplification and high polysomy (FISH+) was present 
      in a significant fraction of the primary (20%) and recurrent (22%) ovarian 
      carcinomas. On mutational analysis, only one tumor with a silent EGFR mutation 
      was observed, and this was the only carcinoma with high-level amplification. EGFR 
      protein immunoexpression was seen in 28% of primary and 33% of recurrent 
      carcinomas and correlated to amplification in the primary tumors (P = 0.003). In 
      recurrent carcinoma, moderate and strong EGFR expression was associated with 
      amplification (P = 0.034). These molecular events potentially have impact on the 
      responsiveness to EGFR targeting agents in ovarian cancer.
FAU - Stadlmann, Sylvia
AU  - Stadlmann S
AD  - Institute for Pathology, University Hospital of Basel, Basel, Switzerland.
FAU - Gueth, Uwe
AU  - Gueth U
FAU - Reiser, Ulrich
AU  - Reiser U
FAU - Diener, Pierre-Andre
AU  - Diener PA
FAU - Zeimet, Alain Gustave
AU  - Zeimet AG
FAU - Wight, Edward
AU  - Wight E
FAU - Mirlacher, Martina
AU  - Mirlacher M
FAU - Sauter, Guido
AU  - Sauter G
FAU - Mihatsch, Michael J
AU  - Mihatsch MJ
FAU - Singer, Gad
AU  - Singer G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cystadenocarcinoma, Serous/genetics/metabolism/pathology
MH  - ErbB Receptors/analysis/*genetics
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Mutation
MH  - Neoplasm Recurrence, Local
MH  - Ovarian Neoplasms/genetics/metabolism/*pathology
MH  - Tissue Array Analysis
EDAT- 2006/03/24 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/03/24 09:00
PHST- 2006/03/24 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/03/24 09:00 [entrez]
AID - S0893-3952(22)03391-9 [pii]
AID - 10.1038/modpathol.3800575 [doi]
PST - ppublish
SO  - Mod Pathol. 2006 Apr;19(4):607-10. doi: 10.1038/modpathol.3800575.