PMID- 1655663
OWN - NLM
STAT- MEDLINE
DCOM- 19911121
LR  - 20220408
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 49
IP  - 4
DP  - 1991 Oct 21
TI  - Sero-epidemiological investigations on human herpesvirus 6 (HHV-6) infections 
      using a newly developed early antigen assay.
PG  - 551-7
AB  - Monoclonal antibodies (MAbs) were developed against immunodominant HHV-6 (GS 
      isolate) late and early proteins. The major late protein was identified as a 
      probable glycoprotein with a molecular weight of approximately 110 kDa (gp 110). 
      Immunoblotting of the early antigen yielded proteins of 41 and 38 kDa (p41/38). 
      The MAb to the early protein reacted with cells infected with 14 different HHV-6 
      isolates. In contrast, the MAb against the late protein reacted with only 10 of 
      these isolates, indicating that there was strain variation in this glycoprotein. 
      The percentage of antibody-positive sera reactive with gp110 in the ELISA ranged 
      from 56% to 96% among the different serum donor categories. In contrast, only 
      10-30% of the sera were positive for antibodies to p41/38 with the exception of 
      sera from patients with African Burkitt's lymphoma (ABL) and Hodgkin's disease 
      (HD). These antibody patterns denote the presence of active HHV-6 replication in 
      patients with ABL and HD.
FAU - Iyengar, S
AU  - Iyengar S
AD  - Department of Microbiology, Georgetown University Medical Center Washington, DC.
FAU - Levine, P H
AU  - Levine PH
FAU - Ablashi, D
AU  - Ablashi D
FAU - Neequaye, J
AU  - Neequaye J
FAU - Pearson, G R
AU  - Pearson GR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - AIDS-Related Complex/microbiology
MH  - Adult
MH  - *Antibodies, Monoclonal
MH  - Antigens, Viral/analysis/*immunology
MH  - Burkitt Lymphoma/microbiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fatigue Syndrome, Chronic/microbiology
MH  - Fluorescent Antibody Technique
MH  - Herpesviridae Infections/*diagnosis/epidemiology
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Hodgkin Disease/microbiology
MH  - Humans
MH  - Infectious Mononucleosis/microbiology
MH  - Molecular Weight
MH  - Reference Values
MH  - Serologic Tests
MH  - Viral Proteins/analysis/immunology
EDAT- 1991/10/21 00:00
MHDA- 1991/10/21 00:01
CRDT- 1991/10/21 00:00
PHST- 1991/10/21 00:00 [pubmed]
PHST- 1991/10/21 00:01 [medline]
PHST- 1991/10/21 00:00 [entrez]
AID - 10.1002/ijc.2910490413 [doi]
PST - ppublish
SO  - Int J Cancer. 1991 Oct 21;49(4):551-7. doi: 10.1002/ijc.2910490413.