PMID- 16556763
OWN - NLM
STAT- MEDLINE
DCOM- 20060724
LR  - 20061115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 147
IP  - 7
DP  - 2006 Jul
TI  - Avian MyoD and c-Jun coordinately induce transcriptional activity of the 
      3,5,3'-triiodothyronine nuclear receptor c-ErbAalpha1 in proliferating myoblasts.
PG  - 3408-18
AB  - Although physical interactions with other receptors have been reported, 
      heterodimeric complexes of T(3) nuclear receptors (TR) with retinoid X receptors 
      (RXRs) are considered as major regulators of T(3) target gene expression. 
      However, despite the potent T(3) influence in proliferating myoblasts, RXR 
      isoforms are not expressed during proliferation, raising the question of the 
      nature of the complex involved in TRalpha transcriptional activity. We have 
      previously established that c-Jun induces TRalpha1 transcriptional activity in 
      proliferating myoblasts not expressing RXR. This regulation is specific to the 
      muscle lineage, suggesting the involvement of a muscle-specific factor. In this 
      study, we found that MyoD expression in HeLa cells stimulates TRalpha1 activity, 
      an influence potentiated by c-Jun coexpression. Similarly, in the absence of RXR, 
      MyoD or c-Jun overexpression in myoblasts induces TRalpha1 transcriptional 
      activity through a direct repeat 4 or an inverted palindrome 6 thyroid hormone 
      response element. The highest rate of activity was recorded when c-Jun and MyoD 
      were coexpressed. Using c-Jun-negative dominants, we established that MyoD 
      influence on TRalpha1 activity needs c-Jun functionality. Furthermore, we 
      demonstrated that TRalpha1 and MyoD physically interact in the hinge region of 
      the receptor and the transactivation and basic helix loop helix domains of MyoD. 
      RXR expression (spontaneously occurring at the onset of myoblast differentiation) 
      in proliferating myoblasts abrogates these interactions. These data suggest that 
      in the absence of RXR, TRalpha1 transcriptional activity in myoblasts is mediated 
      through a complex including MyoD and c-Jun.
FAU - Busson, Muriel
AU  - Busson M
AD  - Unite d'Endocrinologie Cellulaire, Unite Mixte de Recherche Differenciation 
      Cellulaire et Croissance, Institut National de la Recherche Agronomique, 2 place 
      Viala, 34060 Montpellier Cedex 1, France.
FAU - Daury, Laetitia
AU  - Daury L
FAU - Seyer, Pascal
AU  - Seyer P
FAU - Grandemange, Stephanie
AU  - Grandemange S
FAU - Pessemesse, Laurence
AU  - Pessemesse L
FAU - Casas, Francois
AU  - Casas F
FAU - Wrutniak-Cabello, Chantal
AU  - Wrutniak-Cabello C
FAU - Cabello, Gerard
AU  - Cabello G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060323
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (MyoD Protein)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Retinoid X Receptor alpha)
RN  - 0 (Thyroid Hormone Receptors alpha)
RN  - 5817-39-0 (Triiodothyronine, Reverse)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/*metabolism
MH  - Cell Proliferation
MH  - *Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - MyoD Protein/metabolism/*physiology
MH  - Myoblasts/*cytology
MH  - Proto-Oncogene Proteins c-jun/metabolism/*physiology
MH  - Quail
MH  - Receptors, Thyroid Hormone/*metabolism
MH  - Retinoid X Receptor alpha/metabolism
MH  - Thyroid Hormone Receptors alpha/*biosynthesis
MH  - *Transcription, Genetic
MH  - Triiodothyronine, Reverse/*metabolism
EDAT- 2006/03/25 09:00
MHDA- 2006/07/25 09:00
CRDT- 2006/03/25 09:00
PHST- 2006/03/25 09:00 [pubmed]
PHST- 2006/07/25 09:00 [medline]
PHST- 2006/03/25 09:00 [entrez]
AID - en.2006-0101 [pii]
AID - 10.1210/en.2006-0101 [doi]
PST - ppublish
SO  - Endocrinology. 2006 Jul;147(7):3408-18. doi: 10.1210/en.2006-0101. Epub 2006 Mar 
      23.