PMID- 1655776
OWN - NLM
STAT- MEDLINE
DCOM- 19911114
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 266
IP  - 29
DP  - 1991 Oct 15
TI  - Endogenous basic fibroblast growth factor-dependent induction of collagenase and 
      interleukin-6 in tumor necrosis factor-treated human microvascular endothelial 
      cells.
PG  - 19162-5
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been shown to enhance the synthesis 
      of interleukin-6 (IL-6) and collagenase in human omental microvascular 
      endothelial (HOME) cell (Mawatari, M., Kohno, K., Mizoguchi, H., Matsuda, T., 
      Asoh, K., Van Damme, J. V., Welgus, H. G., and Kuwano, M. (1989) J. Immunol. 143, 
      1619-1627). In the present study, we have examined whether the TNF-alpha-induced 
      synthesis of IL-6 or collagenase in HOME cells is mediated by an inducible growth 
      factor. Among several growth factors examined, we found that the expression of 
      basic fibroblast growth factor (bFGF) mRNA was the one most prominently enhanced 
      when HOME cells were treated with TNF-alpha. The increase of bFGF mRNA by 
      TNF-alpha in HOME cells was observed in both a dose- and time-dependent manner 
      when assayed by Northern blot analysis. The induction of bFGF mRNA was observed 
      by 3 h after incubation with TNF-alpha, and the maximal increase of 5-fold was 
      obtained after 12 h of incubation with 100 units/ml TNF-alpha. Western blot 
      analysis confirmed the enhanced synthesis of bFGF by TNF-alpha. Metabolic 
      labeling and immunoprecipitation assays of bFGF showed that exposure to TNF-alpha 
      enhanced secretion of bFGF into culture medium and also that TNF-alpha stimulated 
      the production of bFGF molecules with molecular masses of 18, 21, and 22.5 kDa in 
      HOME cells. TNF-alpha induced the expression of collagenase mRNA and IL-6 mRNA in 
      HOME cells as well, and the coadministration of neutralizing anti-bFGF antibody 
      almost completely blocked the effects of TNF-alpha. The treatment of HOME cells 
      with exogenous bFGF significantly stimulated the expression of collagenase mRNA 
      and IL-6 mRNA in HOME cells. Therefore, the biological effects of TNF-alpha on 
      HOME cells may be mediated, at least in part, by TNF-alpha-induced bFGF.
FAU - Okamura, K
AU  - Okamura K
AD  - Department of Biochemistry, Oita Medical School, Japan.
FAU - Sato, Y
AU  - Sato Y
FAU - Matsuda, T
AU  - Matsuda T
FAU - Hamanaka, R
AU  - Hamanaka R
FAU - Ono, M
AU  - Ono M
FAU - Kohno, K
AU  - Kohno K
FAU - Kuwano, M
AU  - Kuwano M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 3.4.24.3 (Microbial Collagenase)
SB  - IM
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Endothelium, Vascular/cytology/drug effects/*metabolism
MH  - Enzyme Induction
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Microbial Collagenase/*biosynthesis/genetics
MH  - Precipitin Tests
MH  - RNA, Messenger/biosynthesis
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/10/15 00:00
MHDA- 1991/10/15 00:01
CRDT- 1991/10/15 00:00
PHST- 1991/10/15 00:00 [pubmed]
PHST- 1991/10/15 00:01 [medline]
PHST- 1991/10/15 00:00 [entrez]
AID - S0021-9258(18)54976-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1991 Oct 15;266(29):19162-5.