PMID- 16563391
OWN - NLM
STAT- MEDLINE
DCOM- 20060504
LR  - 20220129
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 580
IP  - 8
DP  - 2006 Apr 3
TI  - Catalytic folding of the Cepsilon3 domain by its high affinity receptor.
PG  - 2129-34
AB  - The interaction of immunoglobulin E (IgE) with its cellular receptor 
      FcepsilonRIalpha is a central regulator of allergy. Structural studies have 
      identified the third domain (Cepsilon3) of the constant region of epsilon heavy 
      chain as the receptor binding region. The isolated Cepsilon3 domain is a "molten 
      globule" that becomes structured upon binding of the FcepsilonRIalpha ligand. In 
      this study, fluorescence and nuclear magnetic resonance spectroscopies are used 
      to characterise the role of soluble FcepsilonRIalpha in the folding of the 
      monomeric Cepsilon3 domain of IgE. Soluble FcepsilonRIalpha is shown to display 
      characteristic properties of a catalyst for the folding of Cepsilon3, with the 
      rate of Cepsilon3 folding being dependent on the concentration of the receptor.
FAU - Harwood, Naomi E
AU  - Harwood NE
AD  - Laboratory of Molecular Biophysics, Department of Biochemistry, University of 
      Oxford, South Parks Road, Oxford OX1 3QU, UK.
FAU - Price, Nicholas C
AU  - Price NC
FAU - McDonnell, James M
AU  - McDonnell JM
LA  - eng
GR  - G0001352/MRC_/Medical Research Council/United Kingdom
GR  - G0200486/MRC_/Medical Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060315
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Anilino Naphthalenesulfonates)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin epsilon-Chains)
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Proteins)
RN  - 1137-00-4 (7-azatryptophan)
RN  - 630I4V6051 (1-anilino-8-naphthalenesulfonate)
RN  - 8DUH1N11BX (Tryptophan)
SB  - IM
MH  - Anilino Naphthalenesulfonates/chemistry
MH  - Animals
MH  - Catalysis
MH  - Fluorescence
MH  - Humans
MH  - Immunoglobulin Fc Fragments/metabolism
MH  - Immunoglobulin epsilon-Chains/*chemistry/*metabolism
MH  - Mice
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - *Protein Folding
MH  - Protein Structure, Tertiary
MH  - Receptors, IgE/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Spectrometry, Fluorescence
MH  - Time Factors
MH  - Tryptophan/analogs & derivatives/chemistry
EDAT- 2006/03/28 09:00
MHDA- 2006/05/05 09:00
CRDT- 2006/03/28 09:00
PHST- 2005/09/15 00:00 [received]
PHST- 2006/03/01 00:00 [revised]
PHST- 2006/03/01 00:00 [accepted]
PHST- 2006/03/28 09:00 [pubmed]
PHST- 2006/05/05 09:00 [medline]
PHST- 2006/03/28 09:00 [entrez]
AID - S0014-5793(06)00322-X [pii]
AID - 10.1016/j.febslet.2006.03.021 [doi]
PST - ppublish
SO  - FEBS Lett. 2006 Apr 3;580(8):2129-34. doi: 10.1016/j.febslet.2006.03.021. Epub 
      2006 Mar 15.