PMID- 16567189
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20060328
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 41
IP  - 4
DP  - 2006 Apr
TI  - Extracellular matrix dynamics associated with tissue-engineered intravascular 
      sclerotherapy.
PG  - 757-62
AB  - BACKGROUND: The extracellular dynamics after intravascular sclerotherapy with an 
      injectable, fibroblast-based engineered construct is unknown. METHODS: Rabbits 
      underwent ethanol sclerotherapy of a jugular vein segment. Control animals (n = 
      40) underwent no further treatment or an acellular collagen hydrogel was 
      injected. Experimental animals (n = 20) received a tissue-engineered construct. 
      After 1, 2, 4, and 20 to 24 weeks, segments were evaluated for collagen, 
      glycosaminoglycan (GAG), matrix metalloproteinase (MMP) 2 and 9, and tissue 
      inhibitors of MMP (TIMPs) 1 and 2 and scored on a scale of 0 to 3. Groups and 
      time points were compared using nonparametric statistical analysis. RESULTS: 
      Collagen content was higher in animals that received fibroblasts (P < .05). 
      Glycosaminoglycan analysis showed a higher grade only at 1 week (P < .05). 
      Collagen and GAG deposition were prominent at weeks 1 through 4, and decreased 
      over time. Both MMP-2 and MMP-9 and TIMP-1 and TIMP-2 grade decreased with time 
      (P < .01) in all groups, with no differences between groups. CONCLUSION: 
      Enhancement of intravascular sclerotherapy by tissue engineering stems, at least 
      in part, from increased local deposition of collagen and GAG. MMP and TIMPs may 
      play a role in recanalization after experimental sclerotherapy. Tissue 
      engineering may be a valuable adjunct for the treatment of vascular 
      malformations.
FAU - Vogel, Adam M
AU  - Vogel AM
AD  - Department of Surgery, Children's Hospital Boston and Harvard Medical School, 
      Boston, MA 02115, USA.
FAU - Smithers, C Jason
AU  - Smithers CJ
FAU - Kozakewich, Harry P
AU  - Kozakewich HP
FAU - Zurakowski, David
AU  - Zurakowski D
FAU - Moses, Marsha A
AU  - Moses MA
FAU - Burrows, Patricia E
AU  - Burrows PE
FAU - Fauza, Dario O
AU  - Fauza DO
FAU - Fishman, Steven J
AU  - Fishman SJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Hydrogels)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Collagen/*administration & dosage
MH  - Extracellular Matrix/*metabolism
MH  - Fibroblasts/*transplantation
MH  - Hydrogels/*administration & dosage
MH  - Injections
MH  - Matrix Metalloproteinases/*metabolism
MH  - Rabbits
MH  - Sclerotherapy/*methods
MH  - *Tissue Engineering
EDAT- 2006/03/29 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/03/29 09:00
PHST- 2006/03/29 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/03/29 09:00 [entrez]
AID - S0022-3468(06)00110-2 [pii]
AID - 10.1016/j.jpedsurg.2006.02.021 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2006 Apr;41(4):757-62. doi: 10.1016/j.jpedsurg.2006.02.021.