PMID- 16567349
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20151119
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 21
IP  - 2
DP  - 2006 Mar
TI  - Relationship between genotoxicity biomarkers in somatic and germ cells: findings 
      from a biomonitoring study.
PG  - 149-52
AB  - A biomonitoring study to evaluate chromosome and DNA damage respectively in 
      somatic and germ cells of a group of male workers exposed to styrene by using 
      biomarkers of genotoxicity was carried out. Styrene-exposed workers from three 
      different areas of Tuscany and healthy subjects, of comparable mean age, sex and 
      lifestyle characteristics, as a control group not exposed to chemicals, have been 
      enrolled. In addition to previous reports [L. Migliore, A. Naccarati, A. Zanello, 
      R. Scarpato, L. Bramanti and M. Mariani (2002) Hum. Reprod., 17, 2912-2918; L. 
      Migliore, A. Naccarati, F. Coppede et al. (2006) Pharmacogenet. Genomics, 16, 
      87-99] we present now data on a cross-sectional investigation involving a 
      homogeneous group of subjects for which data on both somatic and germ cells have 
      been obtained from individuals (42 exposed and 25 controls). Somatic cell 
      genotoxicity was assessed by analysing the frequency of micronucleated 
      binucleated cells (MNBN) in blood lymphocytes. The micronucleus assay was coupled 
      with centromeric fluorescence in situ hybridization (FISH) analysis. Primary DNA 
      damage in germ cells was evaluated by alkaline single-cell gel electrophoresis 
      (Comet assay) and the percentage of the tail DNA (%TD) was used as parameter of 
      Comet evaluation. Moreover, to investigate the frequencies of aneuploidy and 
      diploidy in sperm, we performed multicolour FISH, using DNA probes specific for 
      the centromeric regions of sex chromosomes and chromosome 2, in decondensed sperm 
      nuclei of samples with normal semen parameters in a subgroup of individuals. 
      Mandelic and phenylglyoxylic acids (MAPGA) in end of shift samples were 
      determined as biomarkers of internal dose. MAPGA excretion was consistent with an 
      exposure to styrene above the threshold limit value-time weighted average 
      concentration of 20 p.p.m. Styrene workers showed significantly higher frequency 
      of MNBN as compared to controls (13.8 +/- 5.2 versus 6.2 +/- 5.1; P < 0.001), due 
      to higher proportions of both micronuclei (MN) arising from chromosomal breakage 
      (C-MN) and harbouring whole chromosomes (C+MN). DNA damage in sperm cells was 
      also higher among styrene-exposed, the %TD being 11.02 +/- 2.99 versus 7.42 +/- 
      2.30 in controls (P < 0.001). The incidence of aneuploidy and diploidy for the 
      tested chromosomes in sperm did not show a statistically significant difference 
      between workers and controls. However, a positive correlation was found between 
      genotoxic damage detected in somatic and in germ cells, even after removing the 
      effect of age (r = 0.475; P < 0.001). Although cytogenetic biomarkers detected 
      both in somatic and germ cells were interrelated, no relationships were apparent 
      with exposure parameters. Styrene exposure may increase the likelihood of both 
      chromosome and DNA damage in somatic and germ cells, thus supporting the 
      hypothesis of an interference on reproductive capacity among exposed workers. 
      This is the first time that a field study shows a correlation between two 
      biomarkers of genotoxicity evaluated at the same time in somatic and germ cells.
FAU - Migliore, L
AU  - Migliore L
AD  - Department of Human and Environmental Sciences, Section of Genetics, University 
      of Pisa, Via S. Giuseppe 22, 56100 Pisa, Italy. l.migliore@geog.unipi.it
FAU - Colognato, R
AU  - Colognato R
FAU - Naccarati, A
AU  - Naccarati A
FAU - Bergamaschi, E
AU  - Bergamaschi E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060327
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Biomarkers)
RN  - 0 (Styrenes)
SB  - IM
MH  - *Biomarkers
MH  - Chromosomes/ultrastructure
MH  - Comet Assay
MH  - Cytogenetics
MH  - *DNA Damage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/metabolism
MH  - Male
MH  - Micronucleus Tests/*methods
MH  - Occupational Exposure
MH  - Spermatozoa/metabolism
MH  - Styrenes/chemistry
EDAT- 2006/03/29 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/03/29 09:00
PHST- 2006/03/29 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/03/29 09:00 [entrez]
AID - gel012 [pii]
AID - 10.1093/mutage/gel012 [doi]
PST - ppublish
SO  - Mutagenesis. 2006 Mar;21(2):149-52. doi: 10.1093/mutage/gel012. Epub 2006 Mar 27.