PMID- 16567367
OWN - NLM
STAT- MEDLINE
DCOM- 20061017
LR  - 20141120
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 12
IP  - 8
DP  - 2006 Aug
TI  - Histamine uptake by human endometrial cells expressing the organic cation 
      transporter EMT and the vesicular monoamine transporter-2.
PG  - 483-9
AB  - Cellular reuptake of monoamines, which is mediated by cell membrane transporters, 
      is followed by accumulation in vesicles by vesicular monoamine transporters 
      (VMAT). The aim of this study was to demonstrate the presence of functional 
      monoamine transporters with high affinity for histamine in human endometrial 
      tissue, since histamine has been implicated as a paracrine signal during 
      endometrial decidualization and embryo implantation. In situ hybridization with 
      (35)S-labelled cRNA probes was used for detection of the organic cationic 
      transporter-2 (OCT-2), the extraneuronal monoamine transporter (EMT), and VMAT-2 
      in cryosections of normal human endometrial tissue. To identify functional 
      transporters for histamine in endometrial cells, we incubated primary cultures of 
      stromal cells and cultures of attached glands with (3)H-labelled histamine. 
      Cultures were pretreated with either corticosterone, a specific inhibitor of EMT, 
      or reserpine, a specific inhibitor of VMAT-2. EMT mRNA was localized in the 
      stroma with peak expression in the secretory phase, whereas OCT-2 mRNA was 
      expressed by few cells in the stroma throughout the cycle. VMAT-2 mRNA was 
      localized in the stroma during the proliferative phase and in the epithelium 
      during the secretory phase. Thus, EMT and VMAT-2, which both have high affinity 
      for histamine, are strongly expressed in endometrial cells. Both corticosterone 
      and reserpine significantly reduced the uptake of (3)H-histamine in stromal cells 
      during the proliferative as well as the secretory phase. This indicates the 
      presence of functional EMT and VMAT-2 transporter proteins throughout the cycle, 
      even though their periods of maximal mRNA expression were limited. The results of 
      uptake experiments with glandular epithelial cells confirmed not only the 
      presence of functional VMAT-2 transporter protein in the secretory phase but also 
      the absence of a histamine-specific plasma membrane transporter throughout the 
      cycle. Thus, endometrial tissue contains both plasma membrane and vesicular 
      membrane monoamine transporters with high affinity for histamine. They can 
      potentially influence the reproductive process by the uptake of extracellular 
      histamine and subsequent release on demand.
FAU - Noskova, Vera
AU  - Noskova V
AD  - Department of Obstetrics and Gynecology, Lund University Hospital, Lund, Sweden. 
      vera.noskova@med.lu.se
FAU - Bottalico, Barbara
AU  - Bottalico B
FAU - Olsson, Hanna
AU  - Olsson H
FAU - Ehinger, Anna
AU  - Ehinger A
FAU - Pilka, Radovan
AU  - Pilka R
FAU - Casslen, Bertil
AU  - Casslen B
FAU - Hansson, Stefan R
AU  - Hansson SR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060327
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Organic Cation Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - 820484N8I3 (Histamine)
RN  - 8B1QWR724A (Reserpine)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Corticosterone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Endometrium/drug effects/*metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Histamine/metabolism/*pharmacokinetics
MH  - Humans
MH  - In Situ Hybridization/methods
MH  - Menstrual Cycle/metabolism
MH  - Middle Aged
MH  - Organic Cation Transport Proteins/genetics/*physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reserpine/pharmacology
MH  - Stromal Cells/cytology/drug effects/metabolism
MH  - Tissue Culture Techniques
MH  - Vesicular Monoamine Transport Proteins/genetics/*physiology
EDAT- 2006/03/29 09:00
MHDA- 2006/10/18 09:00
CRDT- 2006/03/29 09:00
PHST- 2006/03/29 09:00 [pubmed]
PHST- 2006/10/18 09:00 [medline]
PHST- 2006/03/29 09:00 [entrez]
AID - gah259 [pii]
AID - 10.1093/molehr/gah259 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2006 Aug;12(8):483-9. doi: 10.1093/molehr/gah259. Epub 2006 Mar 
      27.