PMID- 1657039
OWN - NLM
STAT- MEDLINE
DCOM- 19911212
LR  - 20190718
IS  - 0269-9370 (Print)
IS  - 0269-9370 (Linking)
VI  - 5
IP  - 9
DP  - 1991 Sep
TI  - Reciprocal in vitro interactions between human herpesvirus-6 and HIV-1 Tat.
PG  - 1095-8
AB  - The transactivation induced by human herpesvirus 6 (HHV-6) on the HIV-1 promoter 
      was studied both in the presence and in the absence of the retroviral 
      transactivator protein (Tat) constitutively expressed in Jurkat cells. Jurkat-tat 
      cells were infected with HHV-6, transfected with a plasmid containing HIV-1 long 
      terminal repeat (LTR)/chloramphenicol acetyltransferase (CAT), and CAT assays 
      were performed. HHV-6 infection in the presence of Tat resulted in significantly 
      higher LTR activation than that observed by Tat or HHV-6 alone, indicating that 
      HHV-6 and Tat interact synergically. Furthermore, it was demonstrated that 
      expression of HIV-1 tat inhibits HHV-6 replication, as shown by a 3.6-15.4-fold 
      reduction in infectious yield. We suggest that HHV-6 could trigger HIV 
      reactivation in HIV-seropositive patients which, in turn, could inhibit HHV-6 
      production.
FAU - Di Luca, D
AU  - Di Luca D
AD  - Institute of Microbiology, University of Ferrara, Italy.
FAU - Secchiero, P
AU  - Secchiero P
FAU - Bovenzi, P
AU  - Bovenzi P
FAU - Rotola, A
AU  - Rotola A
FAU - Caputo, A
AU  - Caputo A
FAU - Monini, P
AU  - Monini P
FAU - Cassai, E
AU  - Cassai E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (Gene Products, tat)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Cell Line, Transformed
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - Gene Products, tat/*genetics/physiology
MH  - HIV Infections/microbiology
MH  - HIV Long Terminal Repeat/*genetics/physiology
MH  - HIV Seropositivity
MH  - HIV-1/*genetics/physiology
MH  - Herpesvirus 6, Human/*genetics/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Plasmids/genetics
MH  - Promoter Regions, Genetic/genetics/physiology
MH  - Transcriptional Activation/*genetics/physiology
MH  - Transformation, Genetic/genetics
MH  - Virus Activation/genetics/physiology
MH  - Virus Replication/genetics/physiology
MH  - tat Gene Products, Human Immunodeficiency Virus
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 10.1097/00002030-199109000-00005 [doi]
PST - ppublish
SO  - AIDS. 1991 Sep;5(9):1095-8. doi: 10.1097/00002030-199109000-00005.