PMID- 16571416
OWN - NLM
STAT- MEDLINE
DCOM- 20090611
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 11
DP  - 2005 Nov
TI  - Identification and characterization of HIV-1 epitopes presented by HLA-A*2603: 
      comparison between HIV-1 epitopes presented by A*2601 and A*2603.
PG  - 1155-66
AB  - Human leukocyte antigen (HLA)-A*26 is one of the alleles associated with a slow 
      progression to AIDS. Identification and characterization of HIV-1-specific 
      epitopes presented by this allele are necessary for studies on the 
      immunopathogenesis of AIDS and vaccine development in Asia, where three HLA-A*26 
      subtypes are frequently found. In the present study, we sought to identify 
      HLA-A*2603-restricted HIV-1 epitopes by using reverse immunogenetics and to 
      compare them with HLA-A*2601-restricted ones recently identified. We found that 
      31 of 110 HIV-1 peptides bound to HLA-A*2603 and that only two peptides 
      (Gag169-177 and Env63-72) induced specific CD8+T cells by stimulating peripheral 
      blood mononuclear leukocytes from HIV-1-infected individuals carrying HLA-A*2603. 
      The specific cytotoxic T lymphocyte clones killed HIV-1 recombinant 
      vaccinia-infected cells, indicating that these two peptides were naturally 
      occurring peptides presented by HLA-A*2603. Gag169-177-specific CD8+T cells were 
      frequently detected in both HLA-A*2601+ and -A*2603+ individuals with chronic 
      HIV-1 infection, whereas Env63-72-specific ones were frequently detected only in 
      the HLA-A*2603+ individuals. Gag169-177 peptide bound equally to both HLA-A*26 
      antigens, whereas Env63-72 peptide bound to A*2603 much more strongly than to 
      A*2601. These findings suggest that the relative affinity of these peptides for 
      the HLA-A*26 subtypes determines whether these peptides are recognized as 
      epitopes in HIV-1-infected individuals carrying these alleles.
FAU - Kawashima, Yuka
AU  - Kawashima Y
AD  - Division of Viral Immunology, Center for AIDS Research, Kumamoto University, 
      Kumamoto, Japan.
FAU - Satoh, Manami
AU  - Satoh M
FAU - Oka, Shinichi
AU  - Oka S
FAU - Takiguchi, Masafumi
AU  - Takiguchi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060105
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*26 antigen)
RN  - 0 (HLA-A*26:01 antigen)
RN  - 0 (HLA-A*26:03 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism/virology
MH  - Cell Line, Tumor
MH  - Cytotoxicity Tests, Immunologic
MH  - Epitope Mapping
MH  - Epitopes/chemistry/*immunology/*metabolism
MH  - HIV-1/*immunology
MH  - HLA-A Antigens/*immunology/*metabolism
MH  - HLA-A2 Antigen/*immunology/*metabolism
MH  - Humans
MH  - Mice
MH  - Peptides/immunology/metabolism
MH  - Viral Proteins/immunology/metabolism
EDAT- 2006/03/31 09:00
MHDA- 2009/06/12 09:00
CRDT- 2006/03/31 09:00
PHST- 2005/05/31 00:00 [received]
PHST- 2005/10/26 00:00 [accepted]
PHST- 2006/03/31 09:00 [pubmed]
PHST- 2009/06/12 09:00 [medline]
PHST- 2006/03/31 09:00 [entrez]
AID - S0198-8859(05)00442-8 [pii]
AID - 10.1016/j.humimm.2005.10.015 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 Nov;66(11):1155-66. doi: 10.1016/j.humimm.2005.10.015. Epub 
      2006 Jan 5.