PMID- 1657211
OWN - NLM
STAT- MEDLINE
DCOM- 19911129
LR  - 20190516
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 53
IP  - 4
DP  - 1991 Aug
TI  - Hepatitogenicity of three plaque purified mutants of hepatotropic mouse hepatitis 
      virus, MHV-2.
PG  - 655-61
AB  - Hepatitogenicity of three plaque purified mutant strains of mouse hepatitis 
      virus, designated as MHV-2S, -2M and -2L, isolated from MHV-2 infected 
      SR-CDF1-DBT cells was studied. After intraperitoneal inoculation with 2 x 10(5) 
      PFU of parental MHV-2 and its mutants to 4-week-old female ICR mice, 40% of mice 
      inoculated with MHV-2S and 20% of mice with -2M died in one week, whereas with 
      -2L all mice survived. All mice inoculated with MHV-2 died in 3 days 
      postinoculation (p.i.). Virus titer of the liver of mice inoculated with MHV-2, 
      -2S and -2M reached peaks (MHV-2:10(7) PFU/0.2 g, -2S: 10(5) PFU/0.2 g and -2M: 
      10(6) PFU/0.2 g) at 96 hr p.i., while with -2L a peak titer (10(3) PFU/0.2 g) was 
      shown at 48 hr p.i. Immunofluorescence revealed MHV specific antigen in the liver 
      of MHV-2S infected mice in and around necrotic areas though less extensive than 
      that of parental MHV-2 infected mice. With MHV-2M specific fluorescence was 
      restricted in degenerated hepatocytes in the small necrotic foci. In mice 
      inoculated with MHV-2L only faint fluorescence was detected. Histopathologically, 
      in the liver of MHV-2S infected mice zonal necrosis and cell infiltration were 
      observed. There were spotty necrosis and focal cell infiltration in the liver of 
      MHV-2M infected mice and only small inflammatory foci were seen in MHV-2L 
      infected mice. Large number of extracellular virions were detectable in MHV-2S 
      but not in -2M and -2L infected mice by electron microscopy.
FAU - Goto, N
AU  - Goto N
AD  - Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, 
      Japan.
FAU - Kyo, M
AU  - Kyo M
FAU - Kang, J K
AU  - Kang JK
FAU - Uchida, K
AU  - Uchida K
FAU - Xu, D L
AU  - Xu DL
FAU - Kai, K
AU  - Kai K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
SB  - IM
MH  - Animals
MH  - Endoplasmic Reticulum/microbiology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Golgi Apparatus/microbiology
MH  - Hepatitis, Viral, Animal/*microbiology
MH  - Liver/microbiology/pathology/ultrastructure
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy, Electron
MH  - Murine hepatitis virus/genetics/*pathogenicity/ultrastructure
MH  - Mutation
MH  - Necrosis
MH  - Specific Pathogen-Free Organisms
MH  - Virion/ultrastructure
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 10.1292/jvms.53.655 [doi]
PST - ppublish
SO  - J Vet Med Sci. 1991 Aug;53(4):655-61. doi: 10.1292/jvms.53.655.