PMID- 16573332
OWN - NLM
STAT- MEDLINE
DCOM- 20070426
LR  - 20190513
IS  - 0022-0493 (Print)
IS  - 0022-0493 (Linking)
VI  - 99
IP  - 1
DP  - 2006 Feb
TI  - Identification of two subterranean termite species (Isoptera: Rhinotermitidae) 
      using cellulase genes.
PG  - 123-8
AB  - To identify subterranean termite species, we designed a pair of common primers 
      that amplified 381-bp fragments from cDNAs encoding the endo-beta-1,4-glucanases 
      (EGases) of Coptotermes formosanus Shiraki and Reticulitermes speratus (Kolbe). 
      cDNAs from C. formosanus and R. speratus, and genomic DNA from R. speratus, were 
      amplified by polymerase chain reaction (PCR) by using this primer pair and then 
      cloned and sequenced. Sequences amplified from C.formosanus cDNA displayed 97-99% 
      identity to cDNA encoding the EGase of C. formosanus (CfEG), and 92-94% identity 
      to cDNA encoding the EGase of R. speratus (RsEG). By contrast, cDNA from R. 
      speratus displayed 99-100% identity to RsEG cDNA and 93-94% identity to CfEG 
      cDNA. CfEG and RsEG cDNAs can therefore be used as markers for the identification 
      of these termite species. This is the first report of the successful 
      identification of termite species by using cDNA and genomic DNA sequences of 
      termite origin.
FAU - Itakura, Shuji
AU  - Itakura S
AD  - Department of Applied Biological Chemistry, Kinki University, Naka-Machi, Nara, 
      631-8505, Japan.
FAU - Masuta, Takafumi
AU  - Masuta T
FAU - Tanaka, Hiromi
AU  - Tanaka H
FAU - Enoki, Akio
AU  - Enoki A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Econ Entomol
JT  - Journal of economic entomology
JID - 2985127R
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - EC 3.2.1.4 (Cellulase)
RN  - EC 3.2.1.4 (endoglucanase EGL1)
SB  - IM
MH  - Animals
MH  - Cellulase/*genetics
MH  - DNA Primers/chemistry
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Electrophoresis, Agar Gel
MH  - Gene Order/genetics
MH  - Genes, Insect/genetics
MH  - Isoptera/*classification/enzymology/*genetics
MH  - Polymerase Chain Reaction/methods
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 2006/04/01 09:00
MHDA- 2007/04/27 09:00
CRDT- 2006/04/01 09:00
PHST- 2006/04/01 09:00 [pubmed]
PHST- 2007/04/27 09:00 [medline]
PHST- 2006/04/01 09:00 [entrez]
AID - 10.1093/jee/99.1.123 [doi]
PST - ppublish
SO  - J Econ Entomol. 2006 Feb;99(1):123-8. doi: 10.1093/jee/99.1.123.