PMID- 16573548
OWN - NLM
STAT- MEDLINE
DCOM- 20060623
LR  - 20211203
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 19
IP  - 4
DP  - 2006 Apr
TI  - The down-regulation of IL1alpha and IL6, in monocytes exposed to extracorporeal 
      photopheresis (ECP)-treated lymphocytes, is not dependent on lymphocyte 
      phosphatidylserine externalization.
PG  - 319-24
AB  - Extracorporeal photopheresis (ECP) has been successfully used to treat some 
      inflammatory conditions. Following ECP, lymphocytes become apoptotic and 
      untreated monocytes, exposed to post-ECP lymphocytes, reduce proinflammatory 
      cytokine secretion. This study attempted to establish if this monocyte 
      immunosuppression was linked to phosphatidylserine externalization (detected 
      using Annexin V) on the apoptotic lymphocytes. Using density gradient and 
      magnetic separation, lymphocytes were isolated from three cutaneous T-cell 
      lymphoma and nine chronic graft versus host disease (cGvHD) patients pre-ECP and 
      prior to re-infusion (post-ECP). The collected lymphocytes were cultured 
      overnight and Annexin V levels determined. Peripheral blood was taken from the 
      same patient 20 h later and the monocytes were isolated. The 'fresh' monocytes 
      were introduced to each 20 h pre- and post-ECP lymphocyte culture, stimulated 
      with lipopolysaccharide (LPS) and Brefeldin A and subsequently tested for 
      intracellular tumour necrosis factor alpha, interleukin 1 alpha (IL1alpha), 
      IL1beta, IL6 and IL8. For cGvHD patients, the relative levels of IL1alpha and IL6 
      were reduced in the untreated, LPS-stimulated monocytes exposed to post-ECP 
      lymphocytes. However, the down-regulation of IL1alpha and IL6 did not correlate 
      to levels of Annexin V-positive lymphocytes. ECP-treated lymphocytes can reduce 
      the ability of LPS-stimulated monocytes to produce some proinflammatory 
      cytokines; however, this effect is not dependent on phosphatidylserine 
      externalization.
FAU - Bladon, John
AU  - Bladon J
AD  - Haematology Department, Rotherham General Hospital, South Yorkshire, UK. 
      john.bladon@rothgen.nhs.uk
FAU - Taylor, Peter C
AU  - Taylor PC
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (Annexin A5)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Phosphatidylserines)
SB  - IM
MH  - Annexin A5/metabolism
MH  - Apoptosis
MH  - Cytokines/metabolism
MH  - Down-Regulation
MH  - Graft vs Host Disease/immunology/therapy
MH  - Humans
MH  - Immunosuppression Therapy
MH  - In Vitro Techniques
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-1/*metabolism
MH  - Interleukin-6/*metabolism
MH  - Lymphocytes/cytology/*immunology/*metabolism
MH  - Lymphoma, T-Cell, Cutaneous/immunology/therapy
MH  - Monocytes/*immunology
MH  - Phosphatidylserines/*metabolism
MH  - *Photopheresis
EDAT- 2006/04/01 09:00
MHDA- 2006/06/24 09:00
CRDT- 2006/04/01 09:00
PHST- 2006/04/01 09:00 [pubmed]
PHST- 2006/06/24 09:00 [medline]
PHST- 2006/04/01 09:00 [entrez]
AID - TRI278 [pii]
AID - 10.1111/j.1432-2277.2006.00278.x [doi]
PST - ppublish
SO  - Transpl Int. 2006 Apr;19(4):319-24. doi: 10.1111/j.1432-2277.2006.00278.x.