PMID- 16574073
OWN - NLM
STAT- MEDLINE
DCOM- 20060524
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 343
IP  - 3
DP  - 2006 May 12
TI  - Mu-calpain is involved in the regulation of TNF-alpha-induced matrix 
      metalloproteinase-3 release in a rheumatoid synovial cell line.
PG  - 937-42
AB  - Calpain is secreted by intra-articular synovial cells and degrades the main 
      components of cartilage matrix proteins, proteoglycan, and collagen, causing 
      cartilage destruction. Matrix metalloproteinase-3 (MMP-3) has also been detected 
      in synovial fluid and serum, and is involved in the development and progression 
      of rheumatoid arthritis by degradation of the extracellular matrix and cartilage 
      destruction. To investigate the relationship between calpain and MMP-3 in 
      rheumatic inflammation, we utilized the rheumatic synovial cell line, MH7A. Tumor 
      necrosis factor (TNF-alpha) stimulation-induced increased expression of 
      mu-calpain, m-calpain, and MMP-3 in these cells, as well as the release of 
      calpain and MMP-3 into the culture medium. The calpain inhibitors, ALLN (calpain 
      inhibitor I) and calpeptin, did not affect the intracellular expression of MMP-3, 
      but reduced the secretion of MMP-3 in a concentration-dependent manner. 
      Down-regulation of mu- but not m-calpain by small interfering RNAs abolished 
      TNF-alpha-induced MMP-3 release from the synovial cells. These findings suggest 
      that calpain, particularly mu-calpain, regulates MMP-3 release by rheumatic 
      synovial cells, in addition to exerting its own degradative action on cartilage.
FAU - Morita, Masaji
AU  - Morita M
AD  - Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, 
      1-1 Yanagido, Gifu 501-1194, Japan.
FAU - Banno, Yoshiko
AU  - Banno Y
FAU - Dohjima, Taikoh
AU  - Dohjima T
FAU - Nozawa, Satoshi
AU  - Nozawa S
FAU - Fushimi, Kazunari
AU  - Fushimi K
FAU - Fan, De-Gang
AU  - Fan DG
FAU - Ohno, Takatoshi
AU  - Ohno T
FAU - Miyazawa, Keiji
AU  - Miyazawa K
FAU - Liu, Naxin
AU  - Liu N
FAU - Shimizu, Katsuji
AU  - Shimizu K
LA  - eng
PT  - Journal Article
DEP - 20060309
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (m-calpain)
RN  - EC 3.4.22.- (mu-calpain)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Arthritis, Rheumatoid/*enzymology
MH  - Calpain/antagonists & inhibitors/biosynthesis/*physiology
MH  - Cell Line
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Humans
MH  - Matrix Metalloproteinase 3/*biosynthesis
MH  - RNA Interference
MH  - Synovial Membrane/cytology/*enzymology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Up-Regulation
EDAT- 2006/04/01 09:00
MHDA- 2006/05/25 09:00
CRDT- 2006/04/01 09:00
PHST- 2006/02/03 00:00 [received]
PHST- 2006/02/27 00:00 [accepted]
PHST- 2006/04/01 09:00 [pubmed]
PHST- 2006/05/25 09:00 [medline]
PHST- 2006/04/01 09:00 [entrez]
AID - S0006-291X(06)00466-9 [pii]
AID - 10.1016/j.bbrc.2006.02.155 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 May 12;343(3):937-42. doi: 
      10.1016/j.bbrc.2006.02.155. Epub 2006 Mar 9.