PMID- 16574327
OWN - NLM
STAT- MEDLINE
DCOM- 20060828
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1760
IP  - 7
DP  - 2006 Jul
TI  - Cartilage aggrecan undergoes significant compositional and structural alterations 
      during laryngeal cancer.
PG  - 1046-53
AB  - Aggrecan is a key component of cartilage and is responsible for the integrity and 
      function of the tissue. In this study, the content of aggrecan and its structural 
      modifications in adjacent to cancer apparently normal cartilages (AANCs) from 
      various stages of laryngeal squamous cell carcinoma (LSCC) were investigated. Our 
      data demonstrated a stage-related loss of aggregable aggrecan in AANCs, compared 
      to the healthy laryngeal cartilage (HLC), which was excessive in advanced stages 
      of disease. On aggregable aggrecan level, AANCs were characterized by significant 
      compositional and structural modifications, the extent of which was closely 
      related with the stage of LSCC. Four concrete subpopulations of aggregable 
      molecules with particular physicochemical characteristics were identified with a 
      strong tendency to prevail subpopulations of molecules of lower hydrodynamic 
      sizes with increasing LSCC stage. These findings demonstrated that the cleavage 
      of aggregable aggrecan occurred in concrete peptide bonds within the CS-1 and 
      CS-2 attachment domains. These significant alterations were closely associated 
      with the process of cartilage destruction, indicating the crucial role of 
      aggrecan during LSCC.
FAU - Skandalis, Spyros S
AU  - Skandalis SS
AD  - Laboratory of Biochemistry, Section of Organic Chemistry, Biochemistry and 
      Natural Products, Department of Chemistry, University of Patras, 26500 Patras, 
      Greece.
FAU - Theocharis, Achilleas D
AU  - Theocharis AD
FAU - Vynios, Demitrios H
AU  - Vynios DH
FAU - Papageorgakopoulou, Nickoletta
AU  - Papageorgakopoulou N
FAU - Hjerpe, Anders
AU  - Hjerpe A
FAU - Karamanos, Nikos K
AU  - Karamanos NK
FAU - Theocharis, Dimitrios A
AU  - Theocharis DA
LA  - eng
PT  - Journal Article
DEP - 20060309
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Aggrecans)
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Peptides)
SB  - IM
MH  - Adult
MH  - Aggrecans
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cartilage/*metabolism/pathology
MH  - Chondroitin Sulfate Proteoglycans/*biosynthesis/chemistry
MH  - Extracellular Matrix Proteins/*biosynthesis/chemistry
MH  - *Gene Expression Regulation, Neoplastic
MH  - Glycosaminoglycans/chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*metabolism
MH  - Lectins, C-Type/*biosynthesis/chemistry
MH  - Male
MH  - Middle Aged
MH  - Peptides/chemistry
MH  - Protein Binding
MH  - Protein Structure, Tertiary
EDAT- 2006/04/01 09:00
MHDA- 2006/08/29 09:00
CRDT- 2006/04/01 09:00
PHST- 2005/12/21 00:00 [received]
PHST- 2006/02/08 00:00 [revised]
PHST- 2006/02/08 00:00 [accepted]
PHST- 2006/04/01 09:00 [pubmed]
PHST- 2006/08/29 09:00 [medline]
PHST- 2006/04/01 09:00 [entrez]
AID - S0304-4165(06)00038-9 [pii]
AID - 10.1016/j.bbagen.2006.02.007 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2006 Jul;1760(7):1046-53. doi: 
      10.1016/j.bbagen.2006.02.007. Epub 2006 Mar 9.